Oligomerization of the IHF Protein in the Presence of Metal Cations

The oligomeric state of the nucleoid-associated protein IHF (integration host factor) plays a significant role in the organization and compaction of the bacterial nucleoid and also in the evolution of bacterial resistance to unfavorable environmental conditions, in particular to antibiotics. Althoug...

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Bibliographic Details
Published in:Crystallography reports 2024, Vol.69 (2), p.206-213
Main Authors: Gordienko, A. M., Dadinova, L. A., Petoukhov, M. V., Mozhaev, A. A., Manuvera, V. A., Lazarev, V. N., Shtykova, E. V.
Format: Article
Language:English
Subjects:
Aqueous solutions
Cations
Crystallography and Scattering Methods
Oligomerization
Physics
Physics and Astronomy
Proteins
Structure of Macromolecular Compounds
X-ray scattering
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Summary:The oligomeric state of the nucleoid-associated protein IHF (integration host factor) plays a significant role in the organization and compaction of the bacterial nucleoid and also in the evolution of bacterial resistance to unfavorable environmental conditions, in particular to antibiotics. Although IHF was identified more than 25 years ago, the molecular mechanisms of its participation in these processes are poorly understood. Using small-angle X-ray scattering, it was demonstrated for the first time that there are different oligomeric states of IHF in an aqueous medium depending on the presence of metal cations. It was found that the presence of Mg 2+ and K + ions hinders the formation of high-order oligomers of IHF. The results of this study may be useful in the development of strategies against bacterial resistance to drugs.
ISSN:1063-7745
1562-689X
DOI:10.1134/S1063774524600133