In Vivo Evaluation of purD Gene Deleted Brucella abortus in Mice as Potential Vaccine Candidate for Control of Brucellosis

ABSTRACT Brucella abortus (B. abortus) RB51 is a globally recognized and widely practiced vaccinal strain for the control of bovine brucellosis but has several reported drawbacks including pathogenic potential for humans and excreted in body fluids of vaccinated animals. In the present study, we con...

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Bibliographic Details
Published in:Pakistan journal of zoology 2024-06, Vol.56 (3), p.1271
Main Authors: Riaz, Muhammad Ilyas, Rabbani, Masood, Raza, Sohail, Awan, Ali Raza, Kokab, Aleena, Durrani, Rida Haroon
Format: Article
Language:English
Subjects:
Abortion
Animal sciences
Animals
Attenuated vaccines
Attenuation
Body fluids
Brucella abortus
Brucellosis
Cattle
Cloning
E coli
Enzymes
Fluids
Histopathology
Immune clearance
Immune response
Immunization
Macrophages
Mutagenesis
Mutants
Prevention
Site-directed mutagenesis
Spleen
Splenomegaly
Vaccines
Virulence
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Summary:ABSTRACT Brucella abortus (B. abortus) RB51 is a globally recognized and widely practiced vaccinal strain for the control of bovine brucellosis but has several reported drawbacks including pathogenic potential for humans and excreted in body fluids of vaccinated animals. In the present study, we constructed a more attenuated B. abortus ΔpurD mutant by deleting the purD gene from the RB51 strain through site-directed mutagenesis. For virulence attenuation comparison with the parent RB51 strain, the constructed mutant was evaluated for attenuation estimation and clearance in BALB/c mice. The B. abortus ΔpurD mutant exhibited significant attenuation of virulence and cleared from the mice spleen after 20th DPI when inoculated at the dose of 108 CFU per mice. In contrast to this, the parent RB51 strain induces splenomegaly and showed higher persistence with significantly higher splenic CFUs in the mice group at 10th and 20th DPI as compared to B. abortus ΔpurD mutant. The histopathological comparison of spleens from both groups also revealed much infiltration of giant macrophages in the B. abortus ΔpurD mice group as compared to the parent RB51 mice group indicating superior immune response generation. The findings of this study revealed that the highly attenuated B. abortus ΔpurD mutant can be used effectively as a potential live attenuated vaccine candidate for the control of bovine brucellosis both in local and international settings.
ISSN:0030-9923
0030-9923
DOI:10.17582/journal.pjz/20220901090919