3D printed β-sheet-reinforced natural polymer hydrogel bilayer tissue engineering scaffold

It remains a significant challenge to fabricate natural polymer (NP) hydrogels with anti-swelling ability and high strengths in the physiological environment. Herein, the β-sheet-reinforced NP hydrogel is developed by copolymerizing methacrylated gelatin (GelMA) and methacrylated silk fibroin (SFMA)...

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Bibliographic Details
Published in:Science China. Technological sciences 2024-04, Vol.67 (4), p.1170-1184
Main Authors: Zhao, XinRui, Nie, XiongFeng, Zhang, XiaoPing, Sun, YaGe, Yang, Rong, Bian, XinYu, Zhang, Qian, Wang, HongYing, Xu, ZiYang, Liu, WenGuang
Format: Article
Language:English
Subjects:
Aqueous solutions
Bilayers
Bioglass
Bone marrow
Compressive strength
Copolymerization
Crosslinking
Differentiation (biology)
Engineering
Ethanol
Gelatin
Hydrogels
Mechanical properties
Modulus of elasticity
Natural polymers
Regeneration (physiology)
Scaffolds
Silk fibroin
Stem cells
Tensile strength
Three dimensional printing
Tissue engineering
Toughness
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Summary:It remains a significant challenge to fabricate natural polymer (NP) hydrogels with anti-swelling ability and high strengths in the physiological environment. Herein, the β-sheet-reinforced NP hydrogel is developed by copolymerizing methacrylated gelatin (GelMA) and methacrylated silk fibroin (SFMA) in aqueous solution, followed by ethanol treatment (named GelMA-SFMA-AL). The β-sheets formed by SFMA can act as a stable physical crosslink to enhance the mechanical properties and prolong the degradation of the GelMA network. Importantly, the chemical crosslinking in the GelMA-SFMA hydrogel prevents excessive aggregation of hydrophobic β-sheets, thereby avoiding the formation of brittle hydrogel. The obtained GelMA-SFMA-AL hydrogels exhibit considerably enhanced mechanical properties (Young’s modulus: 0.89–3.68 MPa; tensile strength: 0.31–0.96 MPa; toughness: 0.09–0.63 MJ/m 3 ; compressive modulus: 0.78–2.20 MPa; compressive strength: 2.65–5.93 MPa) compared with GelMA-SFMA hydrogels (Young’s modulus: 0.04–0.13 MPa; tensile strength: 0.04–0.07 MPa; toughness: 0.01–0.02 MJ/m 3 ; compressive modulus: 0.03–0.09 MPa; compressive strength: 0.30–0.64 MPa). A bilayer osteochondral scaffold is constructed via digital light processing (DLP) three-dimensiaonl (3D) printing technology, comprising GelMA-SFMA@diclofenac sodium (DS)-AL as the top layer and GelMA-SFMA@bioactive glass (BG)-AL as the bottom layer. The bilayer hydrogel scaffold is demonstrated to support cell attachment and spreading, and facilitate osteogenic differentiation of rat bone marrow stem cells in vitro . In vivo implantation experiment suggests this bilayer scaffold is promising to be used for osteo-chondral tissue regeneration.
ISSN:1674-7321
1869-1900
DOI:10.1007/s11431-023-2471-0