Mechanism of pterostilbene in improving myocardial injury after percutaneous coronary intervention through being targeted on miR-26a-5p

Our study aimed to discuss the mechanism of pterostilbene in improving myocardial injury after percutaneous coronary intervention (PCI) through targeting miR-26a-5p. The myocardial cells were isolated from C57BL/6 mice. They were frozen in liquid nitrogen for reservation after they were passaged. Th...

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Bibliographic Details
Published in:Materials express 2024-04, Vol.14 (4), p.651-656
Main Authors: Zhao, Lini, Sun, Guowei, Wang, Longtao, Fang, Cao, Chen, Yuewu, Ding, Ping
Format: Article
Language:English
Subjects:
Angioplasty
Flow cytometry
Injuries
Liquid nitrogen
Proteins
Spectrophotometry
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Summary:Our study aimed to discuss the mechanism of pterostilbene in improving myocardial injury after percutaneous coronary intervention (PCI) through targeting miR-26a-5p. The myocardial cells were isolated from C57BL/6 mice. They were frozen in liquid nitrogen for reservation after they were passaged. The cell transfection was performed with miR-26a-5p depressor or lipofectamine 3000. The Ischemia-Reperfusion (I/R) model was established. They were divided into several sets including control set, I/R set, miR-26a-5p imitative set, miR-26a-5p depressor set and pterostilbene set. The presentation of GAPDH and miR-26a-5p was monitored with Real-time PCR. The proliferation was tested with Flow Cytometry (FCM). Caspase-3 activity was tested with spectrophotometry. The protein expression was monitored with Western blot assay. The level of IL-6 and TNF- α was tested with ELISA method. There was abnormal miR-26a-5p expression in the I/R model. The survival rate of myocardial cells was improved by upregulating miR-26a-5p. And expression of apoptotic protein as p53 was reduced and SOD activity was increased. Reactive oxygen species (ROS) level was reduced. The level of IL-6 and TNF- α was restrained. miR-26a-5p in I/R model was increased with pterostilbene notably. The myocardial injury was improved by pterostilbene through regulating miR-26a-5p. It could provide a brand-new scheme for treating myocardial injury after PCI.
ISSN:2158-5849
2158-5857
DOI:10.1166/mex.2024.2634