Internalization of scandium-46 labeled zirconium doped hydroxyapatite in lung cancer cell and in silico study of its mechanism

High-Z ion doped hydroxyapatite, including zirconium doped hydroxyapatite has prospects as drug candidates for x-ray induced dynamic therapy (XDT) in cancer therapy. Its nanosized combined with doped ion could assist ion doped hydroxyapatite accumulates in cancer cell compared to non doped hydroxyap...

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Main Authors: Febrian, Muhamad Basit, Kurniawan, Ahmad, Fikri, Ahsanal, Mahendra, Isa, Wibawa, Teguh Hafiz Ambar, Sulaiman, Sulaiman, Juliyanto, Sumandi, Setiadi, Yanuar, Rattyananda, Badra Sanditya, Dewanti, Maria Rosa, Putra, Amal Rezka
Format: Conference Proceeding
Language:English
Subjects:
Binding energy
Docking
Hydroxyapatite
Kinases
Lung cancer
Proteins
Receptors
Scandium
Therapy
Tyrosine
Zirconium
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Summary:High-Z ion doped hydroxyapatite, including zirconium doped hydroxyapatite has prospects as drug candidates for x-ray induced dynamic therapy (XDT) in cancer therapy. Its nanosized combined with doped ion could assist ion doped hydroxyapatite accumulates in cancer cell compared to non doped hydroxyapatite. However the mechanism of ion doped hydroxyapatite internalization in cancer cell is currentle unknown. In this study, internalization of scandium-46 (46Sc) labeled zirconium doped hydroxyapatite (HApZr) and 46Sc non doped hydroxyapatite (HAp) in A549 lung cancer cell line was carried out to understand the effect of zirconium doping into HAp. Docking study was carried out to compared HApZr and HAp binding energy to several receptor proteins related to internalization including receptor tyrosine kinase of ERBB2 and PI3K/AKT protein kinase B. HApZr was internalized higher in lung cancer compared to HAp. Docking study revealed that binding energy of HApZr with ERBB2 and PI3K protein was lower compared to HAp. ERBB2 and PI3K protein are overexpressed in cancer cell thus could be a key for cancer survival pathway. Lower binding energy of HApZr stimulates higher uptake of HApZr in cancer cell compared to HAp as obtained in internalization study. These findings have important implications for further development of HApZr or other ion doped hydroxyapatite as potential agent for x-ray induced therapy of cancer.
ISSN:0094-243X
1551-7616
DOI:10.1063/5.0193439