Rapid detection of antibiotic sensitivity of Staphylococcus aureus by Raman tweezers

Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of...

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Bibliographic Details
Published in:European physical journal plus 2021-02, Vol.136 (2), p.233, Article 233
Main Authors: Bernatová, S., Rebrošová, K., Pilát, Z., Šerý, M., Gjevik, A., Samek, O., Ježek, J., Šiler, M., Kizovský, M., Klementová, T., Holá, V., Růžička, F., Zemánek, P.
Format: Article
Language:English
Subjects:
Acids
Antibiotics
Antimicrobial agents
Applied and Technical Physics
Atomic
Bacteria
Complex Systems
Condensed Matter Physics
Drug resistance
Etching
Focus Point on Light Pressure across All Scales
Gas flow
Laboratories
Lipids
Mathematical and Computational Physics
Molecular
Nosocomial infections
Nucleic acids
Optical and Plasma Physics
Optical trapping
Penicillin
Phenylalanine
Physics
Physics and Astronomy
Proteins
Raman spectroscopy
Regular Article
Spectrum analysis
Staphylococcus infections
Theoretical
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Summary:Methicillin-resistant Staphylococcus aureus is a bacterium pathogenic to humans and a leading cause of the hospital-acquired infections, causing significant increase in morbidity and mortality. Conventional antibiotic sensitivity testing requires culturing of the isolated pathogen in the presence of antibiotics, and it takes at least 48 hours. Comparatively faster determination of bacterial sensitivity to antibiotics can be achieved with Raman tweezers—an analytical method based on Raman spectroscopy and optical trapping. This article demonstrates the effectiveness of this approach for the discrimination between a methicillin-resistant and a methicillin-sensitive strain of Staphylococcus aureus in about 4 hours from a microliter volume of the bacterial sample. We found that the antibiotic-induced changes in the bacterial cells influenced the ratio of the Raman signals of nucleic acids to phenylalanine. This points to the antibiotic causing cell lysis and the associated loss of nucleic acids from the cytoplasm.
ISSN:2190-5444
2190-5444
DOI:10.1140/epjp/s13360-021-01152-1