Impact of sequential exposure of Beauveria bassiana and imidacloprid against susceptible and resistant strains of Musca domestica

Insecticide resistance in the housefly Musca domestica is hampering pest management. However, entomopathogens, possibly in combination with insecticides, may have control potential against resistant houseflies. This study investigates the combination of the entomopathogenic fungus Beauveria bassiana...

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Bibliographic Details
Published in:BioControl (Dordrecht, Netherlands) Netherlands), 2018-10, Vol.63 (5), p.707-718
Main Authors: Farooq, Muzammil, Steenberg, Tove, Højland, Dorte Heidi, Freed, Shoaib, Kristensen, Michael
Format: Article
Language:English
Subjects:
Agriculture
Animal Biochemistry
Animal Ecology
Beauveria bassiana
Behavioral Sciences
Bioassays
Biomedical and Life Sciences
Combined treatment
Detoxification
Entomology
Entomopathogenic fungi
Enzymes
Experiments
Females
Fungi
Gene expression
Genes
Imidacloprid
Insecticide resistance
Insecticides
Insects
Life Sciences
Mortality
Musca domestica
Pest control
Pesticide resistance
Plant Pathology
Public health
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Summary:Insecticide resistance in the housefly Musca domestica is hampering pest management. However, entomopathogens, possibly in combination with insecticides, may have control potential against resistant houseflies. This study investigates the combination of the entomopathogenic fungus Beauveria bassiana and the neonicotinoid insecticide, imidacloprid against a susceptible and a resistant housefly strain, respectively under laboratory conditions. The fungus and insecticide were tested alone and in combinations at LC 30 . Significant and synergistic interactions between B. bassiana and imidacloprid were observed with increased mortality rates of the combined treatment as compared to individual treatment in housefly strains 772a (susceptible) and 766b (resistant). Significant differences in the GST and P450 activities for both strains were found. Female 766b flies caused 15- to 237-fold increases in gene expression of xenobiotic response genes for B. bassiana and 23- to 120-fold changes for imidacloprid. The combination of B. bassiana and imidacloprid caused significant synergistic interaction when applied against two housefly strains irrespective of order of application. The effect was highest when the insecticide was applied first. The resistant housefly strain had elevated detoxification enzymes and higher expression of detoxification genes, but showed the same level of susceptibility to the combined fungus/insecticide treatment as the susceptible strain.
ISSN:1386-6141
1573-8248
DOI:10.1007/s10526-018-9892-6