A Perfusion Bioreactor for Long-Term Studies of the Dynamics of the Formation of a Tissue Equivalent

This paper presents results obtained from a modified perfusion bioreactor designed to form tissue equivalents in conditions of long-term cultivation with the capacity to oxygenate the medium. The design of the device permits the morphology of cellular engineering structures to be studies at four cul...

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Bibliographic Details
Published in:Biomedical engineering 2022-11, Vol.56 (4), p.221-224
Main Authors: Basok, Yu. B., Grigor’ev, A. M., Kirsanova, L. A., Kirillova, A. D., Sevast’yanov, V. I.
Format: Article
Language:English
Subjects:
Biomaterials
Biomedical and Life Sciences
Biomedicine
Bioreactors
Carbon dioxide
Cartilage
Cell culture
Cellular structure
Cultivation
Culture media
Engineering
Equivalence
Hydrogels
Metabolites
Morphology
Perfusion
Sensors
Sterility
Surgical apparatus & instruments
Yeast
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Summary:This paper presents results obtained from a modified perfusion bioreactor designed to form tissue equivalents in conditions of long-term cultivation with the capacity to oxygenate the medium. The design of the device permits the morphology of cellular engineering structures to be studies at four cultivation time points under flow conditions and also provides for constant monitoring of culture medium pH, O 2 and CO 2 contents, and major metabolite contents throughout the experiment. The functional effectiveness of the bioreactor is confirmed by the example of “growing” the tissue equivalent of cartilage for 25 days with maintenance of cell culture viability, with constant values for the main culture medium parameters and sterility.
ISSN:0006-3398
1573-8256
DOI:10.1007/s10527-022-10206-2