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Effect of ChREBP on carbohydrate feeding regulation in mandarin fish Siniperca chuatsi
Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate. Therefore, the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish, the effective conversion and utilization of carbohydrate and lipids, and the feedback regulation of...
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Published in: | Journal of oceanology and limnology 2023-09, Vol.41 (5), p.1988-1996 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | Carnivorous fish have poor tolerance to carbohydrate in feed and low utilization rate of carbohydrate. Therefore, the balance of carbohydrate and lipids in the nutrient metabolism of carnivorous fish, the effective conversion and utilization of carbohydrate and lipids, and the feedback regulation of feeding are the key links for the efficient utilization of carnivorous fish feed. Carbohydrate response element binding protein (ChREBP) is a new transcription factor found in recent years in the glucose signaling pathway, and can also participate in feeding regulation. We performed in-vivo and in-vitro experiments to reveal the role of ChREBP in the glucose metabolism and feeding in mandarin fish. The mRNA expression of
ChREBP
and appetite regulatory factors were measured after intraperitoneal injection of glucose in mandarin fish
Siniperca chuatsi
and cotransfection with glucose and glucose+siRNA in the hypothalamic cells in mandarin fish. The results reveal that at hour 2 and 4 post intraperitoneal injection with 1 mg/g BW glucose, the blood glucose level of the mandarin fish increased significantly, but food intake decreased significantly, and it also displayed a significantly increased
ChREBP
mRNA expression levels in liver. At hour 4 post injection, hypothalamic
ChREBP
mRNA level was significantly increased, whereas the mRNA expression levels of appetite genes neuropeptide Y (
npy
) and agouti-related peptide (
AgRP
) were decreased significantly. When the glucose concentration was 40 mmol/L, the expression level of
ChREBP
mRNA in mandarin fish hypothalamic cells was significantly up-regulated, but the expression level of appetite gene
npy
mRNA was down-regulated. When siRNA and glucose were co-transfected into mandarin fish brain cells, the expression level of chrebp mRNA was significantly decreased, and the appetite gene
npy
mRNA was significantly increased. The results indicated that glucose regulated food intake through the modulation of appetite gene
npy
by
ChREBP
. |
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ISSN: | 2096-5508 2523-3521 |
DOI: | 10.1007/s00343-022-1356-2 |