Characterisation of Verticillium dahliae by inter-simple sequence repeats identified a virulent subpopulation affecting Australian cotton

Verticillium dahliae is an agricultural phytopathogen of notable importance around the world and is increasingly impacting the billion-dollar Australian cotton industry. In diseased Australian cotton, the VCG classification system of V. dahliae does not adequately reflect the disease symptoms observ...

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Bibliographic Details
Published in:Australasian plant pathology 2022-07, Vol.51 (4), p.409-418
Main Authors: Dadd-Daigle, Pearl, Collins, Damian, Kirkby, Karen, Roser, Sharlene, Lonergan, Peter, Chowdhury, Piklu Roy, Labbate, Maurizio, Chapman, Toni A.
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Cotton
Damage
Ecology
Entomology
Life Sciences
Original Research Article
Pathogenicity
Pathogens
Plant diseases
Plant Pathology
Plant Sciences
Populations
Signs and symptoms
Simple sequence repeats
Verticillium dahliae
Virulence
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Summary:Verticillium dahliae is an agricultural phytopathogen of notable importance around the world and is increasingly impacting the billion-dollar Australian cotton industry. In diseased Australian cotton, the VCG classification system of V. dahliae does not adequately reflect the disease symptoms observed. The non-defoliating VCG2A isolates have caused significant damage, a problem not readily observed overseas, and the defoliating VCG1A isolates have not always caused widespread severe symptoms as expected. This study examined a selection of V. dahliae isolates derived from diseased cotton plants in NSW, Australia, America and Israel and classified them into groups using Inter-Simple Sequence Repeats (ISSR). A subset of these isolates were tested in a pathogenicity assay using cotton plants. The combined results confirmed that Australia has VCG2A isolates capable of causing significant disease (“defoliating-like”) and that when analysed using ISSR, they are distinct from the non-virulent VCG2A populations. Additionally, there is a group of VCG1A isolates that are able to cause significant damage to cotton plants. The ISSR analysis provides a better understanding of the V. dahliae populations circulating within Australia and is a useful tool for diagnostic use, with a potential use for diagnostic confirmation of a causative strain.
ISSN:0815-3191
1448-6032
DOI:10.1007/s13313-022-00865-w