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Healing processes of burn wounds with honey and vaseline as ointment forms: An in-vivo study in Wistar rats
Burn wounds need enhanced healing by using some workable agent for better treatments. The current work aimed to make a comparison between four types of honey and vaseline in ointment forms in-vivo using experimental rats . Several parameters have been evaluated such as wound epithelialization and va...
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Published in: | Materials express 2023-12, Vol.13 (12), p.2042-2048 |
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Main Authors: | , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Burn wounds need enhanced healing by using some workable agent for better treatments. The current work aimed to make a comparison between four types of honey and vaseline in ointment forms
in-vivo using experimental rats
. Several parameters have been evaluated such as wound epithelialization
and vascular endothelial growth factor expression (VEGF) rates for skins. Some biochemical parameters such as nitrites (NO
2
) and fluorescence recovery after photobleaching (FRAP) were detected for serum. TNF-
α
immune response and hydroxyproline contents were also determined
colorimetrically. Microbial infection of the burns was examined against
Pseudomonas aeruginosa
and
Staphylococcus aureus strains
. The wound epithelialization rate among animal groups after 15 days of treatment ranged from 52.13% to 89.58%. The highest VEGF expression rate was
achieved by using the ointment formed by side honey (SIH) at 9.21% followed by pumpkin honey (PUH) at 8.54%, moringa honey (MOH) at 8.11%, and
nigellasativa
honey (NSH) as 7.02%, while vaseline group reported 5.22% VEGF expression. The ointment formed by the NSH rat group detected the
highest NO
2
at the end of the experimental work to reach (0.07
μ
mol/L) followed by SIH (0.06
μ
mol/L), while MOH and PUH groups reported equal values (0.05
μ
mol/L). The FRAP values varied in rat groups from 735.19
μ
mol TE/g (SIH) to 877.89
μ
mol TE/g (NSH). NSH reported the highest value for TNF-
α
immune response at 589.47 U/mL followed by SIH at 560.89 U/mL, while MOH reported the lowest value at 489.58 U/mL. For the microbial infections, the MOH group had the lowest zone of inhibition 5.18 mm, while the
vaseline group reported the highest 19.88 mm against
Pseudomonas aeruginosa
. It was observed that the highest zone of inhibition (19.22 mm) against
Staphylococcus aureus
was for the vaseline group, while the lowest zone of inhibition (5.33 mm) against
Staphylococcus aureus
was for SIH. All honey varieties, especially MOH had better results for NO
2
, TNF-
α
immune response, and successfully inhibited microbial infections
in-vivo
. |
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ISSN: | 2158-5849 2158-5857 |
DOI: | 10.1166/mex.2023.2569 |