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Medicago truncatula-Sinorhizobium meliloti-Fusarium oxysporum Tripartite Interaction Alters Nodulation and Nitrogen Fixation
Plants encounter a myriad of microorganisms at the root-soil interface that can invade with detrimental or beneficial outcomes. Promoting a symbiotic relationship may interfere with the restriction responses to pathogens. In this study, we established a tripartite Medicago truncatula ( Mt )- Sinorhi...
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Published in: | Journal of plant growth regulation 2023-11, Vol.42 (11), p.7151-7163 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | Plants encounter a myriad of microorganisms at the root-soil interface that can invade with detrimental or beneficial outcomes. Promoting a symbiotic relationship may interfere with the restriction responses to pathogens. In this study, we established a tripartite
Medicago truncatula
(
Mt
)-
Sinorhizobium meliloti
(
Sm
)-
Fusarium oxysporum
(
Fo
) interaction to study the effect of the interplay between symbiosis and defense on the symbiosis establishment and efficiency in a resistant (A17) and susceptible (TN1.11) genotype to
Fo
infection. Our results showed that
Sm
induced the expression of allene oxide cyclase 1 and 2 (
MtAoc1
) and (
MtAoc2
) at 6 and 24 h post infection (hpi) in A17 and at 24 hpi in TN1.11. This up-regulation of
MtAoc1
and
MtAoc2
was also observed when co-inoculating TN1.11 genotype with
Fo
and
Sm
. Moreover, in this later treatment the expression level of the gene of the common signaling pathway doesn’t make infection 3 (
DIM3
) was reduced compared to
Sm
-inoculated plants. This reduction of
DIM3
transcripts was concomitant to a decrease in nodule number (NN), nodule fresh weight, and nitrogen-fixing rate (NFR), and to an accumulation of spermine in nodules. This defectiveness in symbiosis parameters, in the co-inoculation treatment, was also observed in A17 genotype, along with an accumulation of phytohormones in leaves and polyamines in nodules. Our results suggest that in the tripartite interaction, the high expression of
MtAoc
and the decrease of
DMI3
transcripts along with the accumulation of phytohormones and polyamines in leaves overlapped with symbiosis establishment and functioning and thus, reduced NN and NFR. |
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ISSN: | 0721-7595 1435-8107 |
DOI: | 10.1007/s00344-023-11004-0 |