Pretreatment of Grape Pomaces and Stalks Using Deep Eutectic Solvents for Succinic Acid Production Integrated in a Biorefinery Concept

Deep eutectic solvents (DES) have been employed for the pretreatment of grape pomace and stalks within a biorefinery concept. Four DES, produced with choline chloride (ChCl) as hydrogen bond acceptor and four carboxylic acids as hydrogen bond donors, namely formic acid (FA), acetic acid (AA), lactic...

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Published in:Waste and biomass valorization 2023-09, Vol.14 (9), p.2857-2872
Main Authors: Filippi, Katiana, Stylianou, Eleni, Pateraki, Chrysanthi, Koutinas, Apostolis, Ladakis, Dimitrios
Format: Article
Language:English
Subjects:
Acetic acid
Acid production
Acids
Bioreactors
Biorefineries
Carboxylic acids
Choline
Engineering
Environment
Environmental Engineering/Biotechnology
Fermentation
Formic acid
Glucan
Grapes
Hemicellulose
Hydrogen bonds
Hydrolysates
Hydrolysis
Industrial Pollution Prevention
Lactic acid
Lignin
Original Paper
Oxalic acid
Polysaccharides
Pretreatment
Recyclability
Refining
Renewable and Green Energy
Solvents
Succinic acid
Sugar
Waste Management/Waste Technology
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Summary:Deep eutectic solvents (DES) have been employed for the pretreatment of grape pomace and stalks within a biorefinery concept. Four DES, produced with choline chloride (ChCl) as hydrogen bond acceptor and four carboxylic acids as hydrogen bond donors, namely formic acid (FA), acetic acid (AA), lactic acid (LA) and oxalic acid (OA), were evaluated considering lignin removal efficiency, polysaccharide hydrolysis efficiency into C5 and C6 sugars, recyclability and reusability. The mixture of ChCl:LA at 1:10 molar ratio, 120°C and 1 h pretreatment, led to 40% lignin removal over four pretreatment cycles. Enzymatic hydrolysis of the remaining solids after the first pretreatment cycle resulted in 92.7% glucan and 36.6% hemicellulose hydrolysis yield. The hydrolysate was used as fermentation feedstock in batch Actinobacillus succinogenes bioreactor cultures leading to 36 g/L succinic acid with a yield of 0.62 g SA per g total sugars and 0.65 g/(L⋅h) productivity. Using 2 kg of grape pomace and stalks in each pretreatment cycle, the succinic acid that could be produced in five consecutive cycles is 200.8 g, 208 g, 204.9 g, 184.5 g and 94.3 g. Graphical Abstract
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-023-02047-1