INVESTIGATION OF PLATELET DEPOSITION ON TITANIUM WITH DIFFERENT HARD MATERIAL COATINGS AND ROUGHNESS VALUES IN A FLOW CHAMBER

Objectives: The formation of thrombi is still a challenge in ventricular assist devices (VADs). This can be caused by the subsequent deposition of proteins and platelets on foreign surfaces. In VADs, the blood contacting components are mainly made of titanium alloys. This study aims to investigate t...

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Bibliographic Details
Published in:International journal of artificial organs 2023-07, Vol.46 (7), p.439
Main Authors: Esslinger, I, Bierewirtz, T, Schulz, I, Froese, V, Lommel, M, Kertzscher, U
Format: Article
Language:English
Subjects:
Blood
Blood platelets
Coatings
Deposition
Diamond-like carbon
Diamond-like carbon films
Flow chambers
Fluorescence
Fluorescence microscopy
Fluorescent dyes
Fluorescent indicators
In vitro methods and tests
Investigations
Niobium
Niobium nitride
Platelets
Polishing
Proteins
Roughness
Shear rate
Statistical analysis
Titanium
Titanium alloys
Titanium base alloys
Titanium nitride
Ventricle
Ventricular assist devices
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Summary:Objectives: The formation of thrombi is still a challenge in ventricular assist devices (VADs). This can be caused by the subsequent deposition of proteins and platelets on foreign surfaces. In VADs, the blood contacting components are mainly made of titanium alloys. This study aims to investigate the potential for platelet deposition of (1) five different hard material coatings on titanium, which are used to prevent surface scratches and (2) titanium with four different degrees of roughness to determine the influence of the degree of polishing. Methods: An in-vitro test bench was developed to optically investigate the deposition of fluorescent labelled platelets on the protein layer of different surfaces. Samples included (1) coatings: Titanium Nitride, Titanium Niobium Nitride, two types of Diamond-Like Carbon and Wolfram Carbid and (2) titanium with Ra values between 0,122µm and 0,021µm. Human whole blood was incubated with Mepacrine (fluorescent dye) and then pumped with a defined shear rate condition through a flow chamber over the samples. The adhered platelets were visualized via inverted fluorescence microscopy. The analyzation of the green values and the binary image of the generated fluorescent picture offers conclusions about the platelet accumulation and the percentage of the covered surface area, respectively. Results: Statistical analysis only showed significant lower potential for platelet deposition for Titanium Nitride compared to titanium without any coating. Furthermore, all rougher surfaces showed significant higher potential for platelet deposition compared to the most polished surface (Ra=0.021µm). Conclusions: It can be concluded that none of these coatings have a higher potential for platelet deposition than uncoated titanium, pointing towards suitability for blood contacting components. Moreover, high efforts for polishing titanium surfaces are shown to be of high importance in terms of platelet deposition.
ISSN:0391-3988
1724-6040