Drouetiella epilithica sp. nov. and Drouetiella lurida (Oculatellaceae, Synechococcales) isolated in the Republic of Korea based on the polyphasic approach

SUMMARY Five strains of Drouetiella (ACKU666, 667, 668, 669 and 670) were isolated from gravels in water, stone monument and coastal mudflat in Korea, and were studied using morphological and molecular traits. All five strains had thin and simple trichomes and exhibited false branching. From these s...

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Bibliographic Details
Published in:Phycological research 2023-07, Vol.71 (3), p.140-153
Main Authors: Kim, Do‐Hyun, Lee, Nam‐Ju, Wang, Hye‐Ryeung, Lim, An Suk, Lee, Ok‐Min
Format: Article
Language:English
Subjects:
16S rRNA
Algae
Coastal morphology
Coastal waters
Color
Colour
Deoxyribonucleic acid
DNA
Drouetiella epilithica
Folding structures
Fungi
Gravel
Helices
ITS
Mud flats
New species
Oculatellaceae
Phylogenetics
Phylogeny
polyphasic
rRNA 16S
rRNA 23S
taxonomy
Thylakoids
Trichomes
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Summary:SUMMARY Five strains of Drouetiella (ACKU666, 667, 668, 669 and 670) were isolated from gravels in water, stone monument and coastal mudflat in Korea, and were studied using morphological and molecular traits. All five strains had thin and simple trichomes and exhibited false branching. From these strains, four strains (ACKU666, 667, 668 and 669) exhibited similar cell lengths with reddish–brown colored cells such as Drouetiella lurida. The 16S rRNA gene phylogeny showed the four strains formed a clade with Drouetiella lurida, and their DNA similarity was calculated to be 99.1–100%. The color of strain ACKU670 appeared to be in bright blue–green color like Drouetiella fasciculata, and their thylakoids showed a parietal arrangement, which is a characteristic feature of the family Oculatellaceae. Strain ACKU670 turned out to be a sister clade to the D. lurida according to the phylogenetic analysis of the 16S rRNA gene. The 16–23S rRNA internal transcribed spacer secondary folding structure (D1–D1′, Box‐B and V3 helices) confirmed the uniqueness of strain ACKU670, therefore indicating differences from the related species. Considering all the results, we described our strain ACKU670 as Drouetiella epilithica sp. nov. in accordance with the International Code of Nomenclature for Algae, Fungi and Plants.
ISSN:1322-0829
1440-1835
DOI:10.1111/pre.12515