RNA-seq analysis reveals an early defense response to tomato leaf curl New Delhi virus in potato cultivar Kufri Bahar

Potatoes in India are very susceptible to apical leaf curl disease, which causes severe symptoms and greater yield losses. Because the majority of potato cultivars are susceptible to the virus, it is crucial to discover sources of resistance and investigate the mechanism of resistance/susceptibility...

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Bibliographic Details
Published in:Functional & integrative genomics 2023-09, Vol.23 (3), p.215, Article 215
Main Authors: Jeevalatha, Arjunan, Siddappa, Sundaresha, Kumar, Ravinder, Tiwari, Rahul Kumar, Lal, Milan Kumar, Sharma, Sanjeev, Chakrabarti, Swarup Kumar, Singh, Bir Pal
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Biochemistry
Bioinformatics
Biomedical and Life Sciences
Cell Biology
Cell cycle
Cell division
Cell signaling
Cultivars
Defense mechanisms
Disease management
Disease resistance
Gene expression
Gene regulation
Genomes
Immune response
Initiation factors
Inoculation
Leaf-curl
Life Sciences
Microbial Genetics and Genomics
Nucleotide sequence
Original Article
Plant diseases
Plant Genetics and Genomics
Potatoes
Proteins
Ribonucleic acid
RNA
Signal transduction
Transcription factors
Transcription initiation
Transcriptomes
Viral infections
Viruses
Zinc finger proteins
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Summary:Potatoes in India are very susceptible to apical leaf curl disease, which causes severe symptoms and greater yield losses. Because the majority of potato cultivars are susceptible to the virus, it is crucial to discover sources of resistance and investigate the mechanism of resistance/susceptibility in potato cultivars. In this study, the gene expression profile of two potato cultivars, Kufri Bahar (resistant) and Kufri Pukhraj (susceptible), varying in their level of resistance to ToLCNDV, was analyzed using RNA-Seq. The Ion ProtonTM system was used to sequence eight RiboMinus RNA libraries from inoculated and uninoculated potato plants at 15 and 20 days after inoculation (DAI). The findings indicated that the majority of differentially expressed genes (DEGs) were cultivar-or time-specific. These DEGs included genes for proteins that interact with viruses, genes linked with the cell cycle, genes for proteins involved in defense, transcription and translation initiation factors, and plant hormone signaling pathway genes. Interestingly, defense responses were generated early in Kufri Bahar, at 15 DAI, which may have impeded the replication and spread of ToLCNDV. This research provides a genome-wide transcriptional analysis of two potato cultivars with variable levels of ToLCNDV resistance. At an early stage, we observed suppression of genes that interact with viral proteins, induction of genes associated with restriction of cell division, genes encoding defense proteins, AP2/ERF transcription factors, and altered expression of zinc finger protein genes, HSPs, JA, and SA pathway-related genes. Our findings add to a greater comprehension of the molecular basis of potato resistance to ToLCNDV and may aid in the development of more effective disease management techniques.
ISSN:1438-793X
1438-7948
DOI:10.1007/s10142-023-01138-5