An ultra-sensitive uric acid second generation biosensor based on chemical immobilization of uricase on functionalized multiwall carbon nanotube grafted palm oil fiber in the presence of a ferrocene mediator

In this work, palm oil fiber (POF) grafted functionalized multiwall carbon nanotube (FMWCNT) decorated ferrocene (Fc) has been drop coated on a platinum electrode (Pt), in which uricase (UOx) has been chemically immobilized for sensitive and selective biosensing of uric acid (UA). Through the use of...

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Bibliographic Details
Published in:Analytical methods 2023-05, Vol.15 (2), p.2456-2466
Main Authors: Deffo, Gullit, Hazarika, Ranjit, Deussi Ngaha, Marcel Cédric, Basumatary, Mwina, Kalita, Shyamali, Hussain, Nayab, Njanja, Evangéline, Puzari, Panchanan, Ngameni, Emmanuel
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Biosensors
Carbon
Chemical speciation
Electrodes
Humans
Immobilization
Metallocenes
Multi wall carbon nanotubes
Nanotubes, Carbon - chemistry
Palm Oil
Platinum
Reproducibility
Reproducibility of Results
Urate oxidase
Urate Oxidase - chemistry
Uric acid
Uric Acid - analysis
Uric Acid - chemistry
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Summary:In this work, palm oil fiber (POF) grafted functionalized multiwall carbon nanotube (FMWCNT) decorated ferrocene (Fc) has been drop coated on a platinum electrode (Pt), in which uricase (UOx) has been chemically immobilized for sensitive and selective biosensing of uric acid (UA). Through the use of EDC/NHS, a stable bioelectrode (UOx/Fc/FMWCNT-POF/Pt) was obtained and characterized by FTIR/ATRIR, XRD, Raman, EA/EDX, TGA, SEM, TEM, CV, EIS, CA, and DPV. Results from DPV showed the rapid response of the developed bioelectrode towards UA (0.185 V) with high sensitivity (41.14 μA mM −1 ) and good limit of detection (19 μM) in the linear range 10-1000 μM. The low value of Michaelis-Menten constant ( k m = 31.364 μM) shows high affinity of the UA towards the enzyme at the electrode surface. The developed biosensor demonstrates good reproducibility, repeatability, and stability with a deviation of less than 2.5%, and was successfully applied for human blood sample analysis. The CA study revealed a fast response time (2 s) of the sensor. The work has pioneered a new addition to the class of tailorable chemical species for biosensor development and proven to be a promising new tool for point of care testing (POCT) applications. UOx immobilized on Fc decorated FMWCNT-palm oil fiber cushion for development of second generation uric acid biosensor.
ISSN:1759-9660
1759-9679
DOI:10.1039/d3ay00053b