Unsteady-State Mass Transfer in Gels with Microorganisms

Hydrogels are promising for use in tissue engineering as matrices for cell incubation and with subsequent use of such biomaterial systems as bioinks suitable for 3D bioprinting. The heat and mass transfer in hydrogel materials based on agarose and gelatin with model microorganisms incubated in the b...

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Bibliographic Details
Published in:Theoretical foundations of chemical engineering 2023-02, Vol.57 (1), p.67-76
Main Authors: Khramtsov, D. P., Sulyagina, O. A., Pokusaev, B. G., Vyazmin, A. V., Nekrasov, D. A.
Format: Article
Language:English
Subjects:
Biomedical materials
Cell growth
Chemistry
Chemistry and Materials Science
Gelatin
Heterogeneity
Hydrogels
Industrial Chemistry/Chemical Engineering
Mass transfer
Microorganisms
Numerical models
Nutrients
Optics
Three dimensional printing
Tissue engineering
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Summary:Hydrogels are promising for use in tissue engineering as matrices for cell incubation and with subsequent use of such biomaterial systems as bioinks suitable for 3D bioprinting. The heat and mass transfer in hydrogel materials based on agarose and gelatin with model microorganisms incubated in the bulk of the gel are studied by the optical methods of spectroscopy and microscopy. The propagation of the fronts of the nutrient medium in pure gelatin hydrogels and mixed hydrogels based on agarose with the addition of gelatin are compared to determine the diffusion properties of the mixed hydrogels that can ensure the delivery of nutrient components to microorganisms. New data are obtained on the degree of heterogeneity in the growth of microorganisms during their bulk incubation under various temperature conditions of incubation, which is necessary to control the properties of bioink in bioprinting. An analytical description of the curve of cell growth in the gel is proposed, and on its basis an expression for determining the cell growth rate is obtained. A numerical model is developed to describe the absorption of nutrients by cells during their growth.
ISSN:0040-5795
1608-3431
DOI:10.1134/S0040579523010074