Genetic stability of cryopreserved ornamental Lilium germplasm

Abstract The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regene...

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Published in:Plant genetic resources: characterization and utilization 2022-02, Vol.20 (1), p.66-68
Main Authors: Song, Jae-young, Yi, Jung-yoon, Bae, Jinjoo, Lee, Jung-ro, Yoon, Mun-sup, Lee, Young-yi
Format: Article
Language:English
Subjects:
Cryopreservation
Cultivars
Genetic diversity
Germplasm
Lilium
Lilium bolanderi
Low level
Morphology
Plantlets
Plants (botany)
Pollen
Stability
Stigmas (botany)
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Summary:Abstract The genus Lilium contains a number of ornamental crop species, which are commercially important in many countries. As they are vegetatively propagated, maintaining genetic stability is essential for their efficient conservation. In this study, we investigated the genetic stability of regenerated plants of three cultivars ( L . bolanderi ‘Lenora’, L. bolanderi ‘Mount Duckling’ and L. bolanderi ‘Mount Dragon’) and one variety ( L. callosum var. flavum ) after cryopreservation, compared with fresh (donor) and non-cryopreserved plants using morphological traits and ISSR markers. No differences in morphological parameters including flower, stigma and pollen colour, floral spots, floral direction or polymorphic bands were observed between control (fresh and non-cryopreserved) and cryopreserved plantlets. In addition, based on the resulting UPGMA dendrogram, the four taxa were divided into different clusters. All cryopreserved, non-cryopreserved and fresh plants in each group could be grouped together in a single cluster with more than 97 or 100% similarity. The results suggest a very low level or the absence of genetic variation in terms of morphological and genetic stability among the plants regenerated after cryopreservation.
ISSN:1479-2621
1479-263X
DOI:10.1017/S147926212200003X