Alternative Nitrogen Source for Producing Crude Extracted IAA and Suitable Method for Enhancing the Germination of Jerusalem Artichoke

Auxin is a useful compound which has an effect on plant root and shoot elongation. Thus, the aim of this study was to determine a suitable medium formula to produce indole-3-acetic acid (IAA). Adding various nitrogen sources such as beef extract, chicken manure solution, soybean residue, yeast extra...

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Bibliographic Details
Published in:Waste and biomass valorization 2023-05, Vol.14 (5), p.1497-1508
Main Authors: Onrit, Natnicha, Boonlue, Sophon, Mongkolthanaruk, Wiyada, Jogloy, Sanun, Riddech, Nuntavun
Format: Article
Language:English
Subjects:
Acetic acid
Casein
Crop residues
Dipping
Elongation
Engineering
Environment
Environmental Engineering/Biotechnology
Germination
Helianthus tuberosus
Indoleacetic acid
Industrial Pollution Prevention
Jerusalem artichokes
Nitrogen
Nitrogen sources
Original Paper
Plant roots
Poultry manure
Renewable and Green Energy
Residues
Seedlings
Shoots
Soybeans
Tryptophan
Waste Management/Waste Technology
Yeasts
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Summary:Auxin is a useful compound which has an effect on plant root and shoot elongation. Thus, the aim of this study was to determine a suitable medium formula to produce indole-3-acetic acid (IAA). Adding various nitrogen sources such as beef extract, chicken manure solution, soybean residue, yeast extract, soybean powder and casein, supplemented with and without inducible substances such as tryptophan, optimized incubation temperature. IAA content in crude extraction was detected by TLC chromatography. Moreover, both the optimum concentration of IAA crude and the most suitable method (spreading or dipping) for application on Jerusalem artichoke were investigated. Five isolates revealed positive results on IAA product. The alternative medium for IAA production in the formula that consisted of soybean residue showed significantly highest contents of substances like IAA product when the rhizobacteria was cultured at 25 and 30 °C. Sixty µg/ml of crude extracted IAA was the optimum concentration, promoting Jerusalem artichoke ( Helianthus tuberosus L.), growth with 100% germination. In addition, the root and shoot lengths exhibited were 3.79 and 7.14 cm, respectively. Dipping seed method was the most effective approach for encouraging seedling development, with a percentage of germination of 86.67 and root and shoot lengths of 4.48 and 5.39 cm, respectively. Our results demonstrated that a simple approach and appropriate concentration of crude extracted IAA could boost Jerusalem artichoke seedling development. Graphical Abstract
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-022-01970-z