Analysis of m6A methylation in skin tissues of different sex Liaoning cashmere goats

N 6 -methyladenosine (m 6 A) is the most frequent internal modification of mRNA and lncRNA in eukaryotes. We used two high-throughput sequencing method, m 6 A-seq and RNA-seq to identify pivotal m 6 A-modified genes in cashmere fineness and fiber growth. 8062 m 6 A peaks were detected by m 6 A-seq,...

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Published in:Animal biotechnology 2023-04, Vol.34 (2), p.310-320
Main Authors: Wang, Yanru, Li, Gaoqian, Zhang, Xinjiang, Zheng, Yuanyuan, Guo, Suling, Guo, Dan, Zhang, Xinghui, Dou, Xingtang, Hui, Taiyu, Yue, Chang, Sun, Jiaming, Guo, Suping, Bai, Zhixian, Cai, Weidong, Fan, Yixing, Wang, Zeying, Bai, Wenlin
Format: Article
Language:English
Subjects:
A methylated genes
A-seq
Cashmere
cashmere fineness
differently m
Eukaryotes
Fineness
Fluorescence
Gene sequencing
Genes
mRNA
N6-methyladenosine
Next-generation sequencing
RNA modification
RNA-seq
Skin
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Summary:N 6 -methyladenosine (m 6 A) is the most frequent internal modification of mRNA and lncRNA in eukaryotes. We used two high-throughput sequencing method, m 6 A-seq and RNA-seq to identify pivotal m 6 A-modified genes in cashmere fineness and fiber growth. 8062 m 6 A peaks were detected by m 6 A-seq, including 2157 upregulated and 6445 downregulated. Furthermore, by comparing m 6 A-modified genes of the male Liaoning Cashmere Goat (M-LCG) and female Liaoning Cashmere Goat (F-LCG) skin tissues, we get 862 differentially expressed m 6 A-modified genes. To identify differently expressed m 6 A genes associated with cashmere fineness, 11 genes were selected for validation using real time fluorescent quantitative PCR in M-LCG and F-LCG. This study provides an acadamic basis on the molecular regulation mechanism of m 6 A modification in cashmere growth process.
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2021.1962897