Hydrogen sulfide alleviates acrylamide-induced testicular toxicity in male rats

Objective Testicular tissues and sperms are typically vulnerable to oxidative stress and inflammation. Despite functioning as a signaling molecule in different physiological and pathological processes, hydrogen sulfide (H 2 S) role in the reproductive system is not fully recognized. This study aimed...

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Published in:Toxicology and environmental health sciences 2023-03, Vol.15 (1), p.41-51
Main Authors: Mokhlis, Hamada Ahmed, Rashed, Mohammed Helmy, Saleh, Ibrahim Ghalib, Eldeib, Mahmoud Gomaa, El-Husseiny, Ahmed A., Khidr, Emad Gamil, Gomaa, Maher H., Gad, Hesham S., Aglan, Ahmed
Format: Article
Language:English
Subjects:
Acrylamide
Animal tissues
Biomedical and Life Sciences
Biomedicine
Catalase
Cytokines
Environmental Health
Glutathione
Hydrogen sulfide
Inflammation
Interleukin 6
L-Lactate dehydrogenase
Lactate dehydrogenase
Lactic acid
Males
Motility
Nitric oxide
Nitric-oxide synthase
Original Article
Oxidative stress
Parameters
Pharmacology/Toxicology
Reproductive system
Sperm
Superoxide dismutase
Testes
Testosterone
Toxicity
Tumor necrosis factor-α
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Summary:Objective Testicular tissues and sperms are typically vulnerable to oxidative stress and inflammation. Despite functioning as a signaling molecule in different physiological and pathological processes, hydrogen sulfide (H 2 S) role in the reproductive system is not fully recognized. This study aimed to assess whether H 2 S could counteract the testicular damage that acrylamide (AC) causes in male rats. Methods Forty male rats were equally divided randomly into four groups: normal control, AC, H 2 S, and AC + H 2 S who received normal saline, 40 mg/Kg of AC, 200 µg/Kg of sodium hydrosulfide NaHS (H 2 S donor), and 40 mg/Kg of AC + 200 µg/Kg of NaHS by intraperitoneal injection for 14 consecutive days, respectively. Body and testes weights, sperm count and motility, lactate dehydrogenase isoenzyme-x (LDH-X), serum testosterone level, oxidative parameters, the expression level of inducible nitric oxide synthase (iNOS), inflammatory cytokines, and histopathological alterations were evaluated. Results The reduction in relative testicular weights, sperm count, and motility served as evidence of the harmful effects of AC. However, these values were reversed when H 2 S and AC were combined. Additionally, AC significantly decreased serum testosterone level, testicular LDH-X activity, superoxide dismutase, catalase, and reduced glutathione. While malondialdehyde, expression levels of iNOS protein and inflammatory cytokines (TNF-α, IL-1β, and IL-6) levels were elevated. Interestingly, the co-administration of H 2 S with AC reversed these values, demonstrating an opposing effect in the previous parameters. Conclusion H 2 S exhibited a protective effect in the rat model of testicular toxicity induced by AC, which may be associated with the suppression of iNOS expression, proinflammatory cytokines, and the inhibition of oxidative stress injury.
ISSN:2005-9752
2233-7784
DOI:10.1007/s13530-022-00156-3