Comparative physical mapping of rice BAC clones linked to resistance genes Glh,Bph-3 and xa-5 in Oryza sativa L. and O. granulata Nees et Arn. ex Watt

Oryza granulata Nees et Arn. ex Watt. is one of the three wild relatives of rice,which are the most valuable for study and utilization in China.In this study,the homology and physical locations of three rice resistance genes,Glh,Bph-3 and xa-5 are comparatively analyzed between O.sativa and O. granu...

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Bibliographic Details
Published in:Chinese science bulletin 2004, Vol.49 (6), p.591-596
Main Author: XIONG, Zhiyong
Format: Article
Language:English
Subjects:
Artificial chromosomes
BAC-FISH
Bacterial artificial chromosomes
Chromosomes
Fluorescence
Fluorescence in situ hybridization
Gene mapping
Genes
Granulation
Homology
Oryza granulata
Oryza sativa
Pachytene
Phylogeny
Physical mapping
Rice
Southern blotting
亚洲栽培稻
有丝分裂
比较物理制图
疣粒野生稻
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Summary:Oryza granulata Nees et Arn. ex Watt. is one of the three wild relatives of rice,which are the most valuable for study and utilization in China.In this study,the homology and physical locations of three rice resistance genes,Glh,Bph-3 and xa-5 are comparatively analyzed between O.sativa and O. granulata by Southern blotting and fluorescence in situ hybridization (FISH).The results of Southern blotting indicate that there exist homologous sequences of the tested RFLP markers in O. granulata.By using three bacterial artificial chromosome (BAC) clones scanned by the tested RFLP as probes, FISH signals are detected on both mitotic and pachytene chromosomes in O.sativa and O.granulata.Dual-color FISH demonstrates that two of the three BAC clones (14E16 and 38J9) are located on the short arm of the same chromosome pair in O. granulata. Additionally, colinearity is shown for the two clones between O.sativa and O.granulata. Another BAC clone 44B4 is located on the end of the short arm of other chromosome pair in these two species.Although the phylogenetic relationship between O.sativa and O.granulata is the most distinct in Oryza and these two species have evidently different biological features and ecological habits, the relative lengths and arm ratios of the detected chromosomes and the relative positions of the tested clone signals on chromosomes in O. granulata are quite similar to those in O. sativa.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1360/03wc0533