A controlled ovarian stimulation procedure suitable for cynomolgus macaques

This study aimed to develop a more suitable ovarian stimulation procedure for cynomolgus macaques (Macaca fascicularis). Macaques were divided into 4 groups, 7AG, 8AG, 7AN, and 8AN, according to the ovarian stimulation procedure administered (i.e., administration of either a gonadotropin-releasing h...

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Bibliographic Details
Published in:Experimental Animals 2022, Vol.71(4), pp.426-432
Main Authors: Shimozawa, Nobuhiro, Iwata, Takeshi, Yasutomi, Yasuhiro
Format: Article
Language:English
Subjects:
Body weight
Chorionic gonadotropin
cynomolgus macaque
Fertilization
Follicle-stimulating hormone
Gametocytes
GnRH analogue
Gonadotropin-releasing hormone
Gonadotropins
Maturation
Metaphase
oocyte
Oocytes
ovarian stimulation
Ovaries
Pituitary (anterior)
Stimulation
Zygotes
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Summary:This study aimed to develop a more suitable ovarian stimulation procedure for cynomolgus macaques (Macaca fascicularis). Macaques were divided into 4 groups, 7AG, 8AG, 7AN, and 8AN, according to the ovarian stimulation procedure administered (i.e., administration of either a gonadotropin-releasing hormone agonist [GnRH-a] or GnRH antagonist [GnRH-ant]) and the number of menstruations (≤ 7 times or ≥ 8 times) in the previous year. In both procedures, oocyte growth and maturation were induced by administration of human follicle-stimulating hormone and human chorionic gonadotropin. The mean numbers of metaphase II mature and metaphase I premature oocytes collected from the 7AG, 8AG, 7AN, and 8AN groups were 12.1 and 10.4, 12.0 and 13.8, 9.1 and 8.3, and 15.5 and 8.8, respectively (P>0.05). The fertilization rates of the 7AN and 8AN groups (85.3% and 74.7%) tended to be higher compared with those in the 7AG and 8AG groups (59.1% and 47.3%; P>0.05). The 8AN group yielded 19.9 zygotes, which was the largest number per macaque, compared with the other three groups. Furthermore, regarding the decreases in body weight between the start of the procedures and the time of oocyte collection, those of the 7AN and 8AN groups were significantly smaller than those of the 7AG and 8AG groups (P
ISSN:1341-1357
1881-7122
DOI:10.1538/expanim.21-0198