Analysis of the Hypothalamic Orexinergic System in Rats with Different Forms of Genetically Determined Epilepsy

The hypothalamic orexigenic system was studied in mature males of two rat strains with a genetic predisposition to various forms of epileptic activity: Krushinsky–Molodkina (KM) rats characterized by audiogenic seizures and WAG/Rij rats with absence epilepsy. In KM rats, as compared to control (Wist...

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Bibliographic Details
Published in:Journal of evolutionary biochemistry and physiology 2022-11, Vol.58 (6), p.1961-1972
Main Authors: Morina, I. Yu, Mikhrina, A. L., Mikhailova, E. V., Vataev, S. I., Hismatullina, Z. R., Romanova, I. V.
Format: Article
Language:English
Subjects:
Animal Physiology
Auditory stimuli
Biochemistry
Biomedical and Life Sciences
Dopamine D1 receptors
Dopamine D2 receptors
Energy balance
Epilepsy
Evolutionary Biology
Experimental Papers
Gene expression
Hypothalamus
Life Sciences
Orexin receptors
Orexins
Seizures
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Summary:The hypothalamic orexigenic system was studied in mature males of two rat strains with a genetic predisposition to various forms of epileptic activity: Krushinsky–Molodkina (KM) rats characterized by audiogenic seizures and WAG/Rij rats with absence epilepsy. In KM rats, as compared to control (Wistar) rats, PCR analysis revealed elevated mRNA expression levels of dopamine receptors D1 and D2. By contrast, in WAG/Rij rats, there were no differences in D1 mRNA expression levels, but the D2 mRNA expression level was significantly lower. KM and WAG/Rij rats shared higher prepro-orexin mRNA levels and orexin-A expression in perifornical area neurons. KM rats exhibited no differences in mRNA expression levels of orexin receptors, while in WAG/Rij rats, the OX2R mRNA expression level was significantly higher compared to Wistar rats. The obtained data are discussed in the context of the brain orexinergic system involvement in the regulation of the dopaminergic system and energy balance of the whole organism during epileptic activity, as well as the likely function of orexins as adaptation factors in pathological conditions.
ISSN:0022-0930
1608-3202
DOI:10.1134/S0022093022060242