Association of Chemokine Genes CXCL9 and CXCL10 Polymorphisms with Tuberculosis in Pakistani Population

ABSTRACT The CXCR3 ligand family, including CXCL9, CXCL10 and CXCL11, play important role in T-cell, natural killer (NK) cell, NKT cell and dendritic cell trafficking. These chemokines were suggested for their use as alternative markers for TB diagnosis, and their possible involvement in disease pat...

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Bibliographic Details
Published in:Pakistan journal of zoology 2022-12, Vol.54 (6), p.2871
Main Authors: Tabassum, Saleha, Zafar, Muneeza, Mirza, Munazza Raza, Choudhary, Muhammad Iqbal, Ul Haq, Ikram, Malik, Imran Riaz, Ansari, Ambreen, Alam, Mehtab
Format: Article
Language:English
Subjects:
Chemokines
CXCL10 protein
CXCL11 protein
CXCR3 protein
Cytokines
Dendritic cells
Development and progression
Disease
Gene polymorphism
Genes
Genetic analysis
Genetic aspects
Genetic polymorphisms
Genotype & phenotype
Granulomas
Health aspects
Hypothyroidism
Immunology
Infections
Ligands
Lymphocytes T
Minority & ethnic groups
Natural killer cells
Neutrophils
Physiological aspects
Polymorphism
Risk factors
Tuberculosis
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Summary:ABSTRACT The CXCR3 ligand family, including CXCL9, CXCL10 and CXCL11, play important role in T-cell, natural killer (NK) cell, NKT cell and dendritic cell trafficking. These chemokines were suggested for their use as alternative markers for TB diagnosis, and their possible involvement in disease pathophysiology. In the present study, we investigated the genetic association and frequency of CXCL9 and CXCL10 genes polymorphisms in TB patients of Pakistani population. A total of 260 study participants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with EPTB (n = 40). Tri and Tetra-ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant association (OR, 2.1 (0.99 to 4.30), p = 0.04) of A allele of rs2276886 with EPTB group as compared to the control. In genetic model analysis, we found strong association (OR, 0.4 (0.181 to 0.94), p = 0.03) of GG genotype with male with EPTB as compared to female in gender stratified dominant model. Furthermore, in context of different ethnic groups of Pakistani subpopulation, we found Punjabi speaking's to be significantly at higher risk (OR, 2.16 (95%CI, 1.0910 to 4.2901), p = 0.02) of developing TB as compared to other ethnic categories. Strong association of CXCL9 polymorphism (rs2276886 G>A) with TB disease in Pakistani population was concluded especially, Punjabi speaking participants were found to have higher risk of developing TB.
ISSN:0030-9923
0030-9923
DOI:10.17582/journal.pjz/20210810130817