Mitochondria and Cell Death in Suspension Culture of Saccharum officinarum L. under Subzero Temperature Treatment

The process of cell death in sugarcane suspension culture ( Saccharum officinarum L.) after exposure to a subzero temperature of –8°С and features of the mitochondria functioning during this period were studied. It was revealed that the cause of cell death after the end of the stress factor action w...

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Bibliographic Details
Published in:Russian journal of plant physiology 2022-12, Vol.69 (6), Article 113
Main Authors: Lyubushkina, I. V., Stepanov, A. V., Fedyaeva, A. V., Fedoseeva, I. V., Pobezhimova, T. P., Grabelnykh, O. I.
Format: Article
Language:English
Subjects:
Apoptosis
ATP synthase
Biomedical and Life Sciences
Cell culture
Cell death
Cytochrome
Cytochrome-c oxidase
Cytochromes
Electron transport
Life Sciences
Metabolism
Mitochondria
Mortality
Oxidative stress
Plant Physiology
Plant Sciences
Respiration
Saccharum officinarum
Subzero temperature
Sugarcane
Suspension culture
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Summary:The process of cell death in sugarcane suspension culture ( Saccharum officinarum L.) after exposure to a subzero temperature of –8°С and features of the mitochondria functioning during this period were studied. It was revealed that the cause of cell death after the end of the stress factor action was the development of oxidative stress and damage to individual components of mitochondrial ETC: subunit II of cytochrome c oxidase and α- and β-subunits of ATP synthase. Under these conditions, the activity of the alternative pathway (AP) of respiration remained at the control level much longer and decreased more slowly than the activity of the cytochrome pathway. The collapse of respiration occurred 6 h after exposure to subzero temperature after the release of cytochrome c from mitochondria. At the same time, the contribution of AP to the respiration of whole cells and isolated mitochondria in the S. officinarum suspension culture increased to 70%. Thus, the functioning of AP contributed to the maintenance of respiratory metabolism in S. officinarum cells after stress treatment and contributed to the survival of almost 20% of cells in culture.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443722060188