A rapid, selective, and ultrasensitive voltammetric and gravimetric protocol for MMP-1 active form detection

In this paper a rapid, selective, and ultrasensitive protocol for the detection of the active form of matrix metalloproteinase-1 (MMP-1), which is a novel predictive and prognostic biomarker, was presented, which might strengthen the current predictive systems. The biosensor construction procedure w...

Full description

Saved in:
Bibliographic Details
Published in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2022-11, Vol.1 (42), p.8696-879
Main Authors: Kowalczyk, Agata, Nisiewicz, Monika K, Kasprzak, Artur, Bamburowicz-Klimkowska, Magdalena, Nowicka, Anna M
Format: Article
Language:English
Subjects:
Biomarkers
Biosensing Techniques - methods
Biosensors
Chemisorption
Dilution
Electrochemical Techniques - methods
Interstitial collagenase
Limit of Detection
Matrix metalloproteinase
Matrix Metalloproteinase 1
Matrix metalloproteinases
Metalloproteinase
Methylene blue
Methylene Blue - chemistry
Receptors
Voltammetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper a rapid, selective, and ultrasensitive protocol for the detection of the active form of matrix metalloproteinase-1 (MMP-1), which is a novel predictive and prognostic biomarker, was presented, which might strengthen the current predictive systems. The biosensor construction procedure was extremely simple, economical, and time-saving, as it involved only the chemisorption step of the voltammetrically active receptor (tripeptide (Cys-Gly-Ile) labeled with methylene blue (MB) and the sealing thiol. The active form of MMP-1 was recognized based on its hydrolytic activity; as a consequence, the receptor fragment (-Ile-MB) was removed from the sensor surface. The biosensors constructed were characterized by a wide dynamic concentration response range (1.0 pg mL −1 -1.0 μg mL −1 ) and a low detection limit (33 fg mL −1 ), especially the biosensor with voltammetric detection, without the amplification step. One of the important advantages of the proposed biosensors is that they can be directly used to analyze the content of the active form of MMP-1 in clinical samples without the dilution step and any other preparation step. The article presents an innovative, simple, voltammetric sensor with an impressively low detection limit for the determination of the active form of MMP-1 in plasma, which does not require biological compounds.
ISSN:2050-750X
2050-7518
DOI:10.1039/d2tb01803a