Triploidy induction in tambaqui (Colossoma macropomum) using thermal shock: fertilization, survival and growth performance from early larval to the juvenile stage

This study assessed the effectiveness of double thermal shock to induce triploidy in tambaqui (Colossoma macropomum) and compared the fertilization, survival, and growth performance of diploid and triploid fish. Freshly fertilized eggs were subjected to heat shock (40 or 42°C) for 2 min and, after t...

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Bibliographic Details
Published in:Journal of applied aquaculture 2022-10, Vol.34 (4), p.989-1004
Main Authors: Silva Júnior, Gabriela, Copatti, Carlos E., Costa, Lucas, Souza, Gustavo, Bonfá, Hugo C., Melo, José F. B.
Format: Article
Language:English
Subjects:
Biological fertilization
Cold shock
Colossoma macropomum
Diploids
Diploidy
Eggs
Fertilization
Fertilization rate
Fish
Flow cytometry
Growth
Hatching
Heat shock
Juveniles
Larvae
Ploidy
Polyploidy
Survival
Thermal shock
triploidization
Triploidy
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Summary:This study assessed the effectiveness of double thermal shock to induce triploidy in tambaqui (Colossoma macropomum) and compared the fertilization, survival, and growth performance of diploid and triploid fish. Freshly fertilized eggs were subjected to heat shock (40 or 42°C) for 2 min and, after that, a cold shock (4°C) for 25 min. Eggs that were not exposed to the heat/cold shock were used as control. Ploidy was assessed by flow cytometry. Larvae (21 days after hatching) treated with thermal shocks displayed reduced fertilization and survival rates. The larvae in the 40/4°C and 42/4°C groups displayed higher growth than the control group. The juveniles (135 days after hatching) in the 42/4°C group showed higher growth than the other groups. The 42/4°C shock induced triploidy in 17.5% of nuclei. The double thermal shock 42/4°C did not induce a high rate of triploidization in tambaqui but produced fish with better growth performance.
ISSN:1045-4438
1545-0805
DOI:10.1080/10454438.2021.1914800