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Overexpression of α-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress
Low temperature stress affects the yield and quality of crops. To adapt to abiotic stress, plant tubulin families play a crucial role to increase cell cold stability. In the present study, our aim was to develop transgenic tobacco by using a cold-responsive SoTUA gene isolated from Saccharum spp. hy...
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Published in: | Sugar tech : an international journal of sugar crops & related industries 2022-12, Vol.24 (6), p.1680-1688 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Low temperature stress affects the yield and quality of crops. To adapt to abiotic stress, plant tubulin families play a crucial role to increase cell cold stability. In the present study, our aim was to develop transgenic tobacco by using a cold-responsive
SoTUA
gene isolated from
Saccharum
spp. hybrid. Based on fluorescent microscope observation and
TUA
gene expression analysis in different tissues,
TUA
gene expression was found mainly in veins. Moreover, a significant increase of
TUA
transcripts and protein under cold stress was detected by quantificational real-time polymerase chain reaction (qRT-PCR) and western blot in the transgenic plants. Results of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activities, and proline, soluble sugar and protein contents indicated oxidative damage is less than wild type (WT). Overexpression of
SoTUA
gene has improved the cold stress tolerance ability in tobacco. |
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ISSN: | 0972-1525 0974-0740 |
DOI: | 10.1007/s12355-022-01118-2 |