Therapeutic efficacy of N‐acetylcysteine and zinc sulphate against di‐(2‐ethylhexyl) phthalate‐induced testicular oxido‐nitrergic stress in male Wistar rat

The therapeutic efficacy of N‐acetylcysteine (NAC) and zinc sulphate on di‐(2‐ethylhexyl) phthalate (DEHP)‐induced testicular oxido‐nitrergic stress in rats was investigated in 36 male Wistar rats (170 ± 10 g) randomly assigned into one of six groups (n = 6). Group 1 (control) received 2.5 ml/kg of...

Full description

Saved in:
Bibliographic Details
Published in:Andrologia 2022-10, Vol.54 (9), p.n/a
Main Authors: Emojevwe, Victor, Nwangwa, Eze Kingsley, Naiho, Alexander Obidike, Oyovwi, Mega Obukohwo, Igiehon, Osarugue, Ogunwole, Eunice, Makinde‐Taylor, Margaret Sope, Ayotomide, Oluwadamilola Ayodele, Akinola, Adeniran Oluwadamilare, Edesiri, Prince Tesi, Oghenetega, Bright Onome, Ovuakporaye, Simon Irikefe
Format: Article
Language:English
Subjects:
Acetylcysteine
Antioxidants
Nitric oxide
N‐acetylcysteine
oxido‐nitregic stress
phthalates
Testes
Thiopental
Zinc
Zinc sulfate
zinc sulphate
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The therapeutic efficacy of N‐acetylcysteine (NAC) and zinc sulphate on di‐(2‐ethylhexyl) phthalate (DEHP)‐induced testicular oxido‐nitrergic stress in rats was investigated in 36 male Wistar rats (170 ± 10 g) randomly assigned into one of six groups (n = 6). Group 1 (control) received 2.5 ml/kg of distilled water for 42 days, while group 2 (vehicle) received 2.5 ml/kg of corn oil for 42 days. Groups 3,4,5, and 6 were administered DEHP (750 mg/kg/day) for 21 days, after which groups 4, 5, and 6 received zinc sulphate (0.5 mg/kg/day), NAC (100 mg/kg/day), and zinc sulphate (0.5 mg/kg/day) + NAC (100 mg/kg/day) for an additional 21 days respectively. After the experimental period, the animals were euthanized by light thiopental sodium, and their testes were carefully dissected out for histological and biochemical assays. The result shows a significant alteration in testicular levels of malondialdehyde, nitric oxide, antioxidant enzymes, total antioxidant capacity, sulphydryl levels, dehydrogenases and testicular architecture following the administration of DEHP. These effects were reversed by coadministration of NAC and zinc sulphate in the study. We therefore concluded that the combined effects of NAC and ZnSO4 effectively improved testicular antioxidant status and reduced testicular nitregic stress, thus improving testicular architecture and functions.
ISSN:0303-4569
1439-0272
DOI:10.1111/and.14508