Biomimetic syntheses of silver nanoparticles using A. ferruginea bark extracts and tenable approaches for developing anti-infectives

Plant-mediated synthesis of silver nanoparticles (AgNPs) has emerged as a feasible alternative to traditional chemical synthesis and its popularity is growing due to its eco-friendly, easy fabrication, and cost-effective features. In this study, aqueous (Aq) and ethanol (E) bark (B) extracts of A. f...

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Bibliographic Details
Published in:Applied nanoscience 2022-09, Vol.12 (9), p.2579-2594
Main Authors: M, Jeevitha, Ravi, Pavithra V., Subramaniyam, Vinodhini, Pichumani, Moorthi, Sripathi, Shubashini K.
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antioxidants
Bark
Biological effects
Biomimetics
Chemical synthesis
Chemistry and Materials Science
Emission analysis
Ethanol
Excitation
Face centered cubic lattice
Feature extraction
Fluorescence
Herbal medicine
Materials Science
Membrane Biology
Nanochemistry
Nanoparticles
Nanotechnology
Nanotechnology and Microengineering
Original Article
Pseudomonas aeruginosa
Silver
Spectral methods
Zeta potential
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Summary:Plant-mediated synthesis of silver nanoparticles (AgNPs) has emerged as a feasible alternative to traditional chemical synthesis and its popularity is growing due to its eco-friendly, easy fabrication, and cost-effective features. In this study, aqueous (Aq) and ethanol (E) bark (B) extracts of A. ferruginea  (AF) are used to synthesize silver nanoparticles (AgNPs) by sunlight method, and the respective AgNPs are named as AFBAqNPs and AFBENPs. The dark violet-colored silver nanoparticles formed are characterized using various spectral techniques (UV–Vis, FTIR, XRD, FE-SEM, EDS, HR-TEM, DLS and Zeta potential). Antioxidant activity is measured using the DPPH technique and quorum sensing screening is done. The UV–Vis spectra of the synthesized AgNPs exhibits an absorption peak between 420 and 457 nm. The fluorescence analysis shows the emission spectrum of 779 and 894 nm at 390 and 450 nm excitation wavelengths for AFBAqNPs and AFBENPs, respectively. The reduction and capping of AgNPs are due to the phytochemicals present in the bark extract. The FTIR and XRD results reveal their crystalline nature and FCC structure. The HR-TEM images divulge the size of AFBAqNPs, and AFBENPs as ~ 19.73 and 12.33 nm in which AFBENPs have a bimodal size and distribution, with smaller spherical particles with the size of 2.60 nm. The size of AFBAqNPs and AFBENPs are 162.2 and 35.8 nm, respectively, according to FE-SEM. The stability measured via zeta potential of AFBAqNPs and AFBENPs is − 26.1 and 5.9 mV correspondingly. The antioxidant activity of AFBAqNPs is higher (64%) when compared to AFBENPs (56%). Anti-quorum sensing activity for both AgNPs is found to be moderate against  Chromobacterium violaceum (ZOI: 7–11 mm)  and   Pseudomonas aeruginosa (ZOI: 11–14 mm). The strong emission peaks observed at different excitation indicated that both AgNPs (AFBENPs: λ extn : 895 nm, AFBAqNPs: (λ extn : 779 nm) are excitation dependent. This is the first report on the synthesis, fluorescence effects and biological applications of nanoparticles from this medicinal plant . The results of this study suggest that the AgNPs from A. ferruginea  can be used to develop new drugs for the control of  P. aeruginosa -related infections, and more research is needed to identify the characteristic molecules.
ISSN:2190-5509
2190-5517
DOI:10.1007/s13204-022-02570-y