Identification of significant marker-trait associations for Fusarium wilt resistance in a genetically diverse core collection of safflower using AFLP and SSR markers

Safflower ( Carthamus tinctorius L.), an oilseed crop, is severely affected by Fusarium oxysporum f. sp. carthami (Foc), a fungus causing Fusarium wilt (FW) resulting in up to 80% yield loss. In the present study, we used a panel of 84 diverse accessions from the composite core collection to perform...

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Bibliographic Details
Published in:Journal of applied genetics 2022-09, Vol.63 (3), p.447-462
Main Authors: Singh, Krishna Nand, Rawat, Sapna, Kumar, Kuldeep, Agarwal, Surekha Katiyar, Goel, Shailendra, Jagannath, Arun, Agarwal, Manu
Format: Article
Language:English
Subjects:
Admixtures
Amplified fragment length polymorphism
Animal Genetics and Genomics
Biomedical and Life Sciences
Carthamus tinctorius
Fusarium
Fusarium oxysporum
Gene mapping
Genetic analysis
Genotyping
Human Genetics
Hydroponics
Life Sciences
Mapping
Markers
Microbial Genetics and Genomics
Oilseed crops
Oilseeds
Phenotypic variations
Plant Genetics and Genomics
Plant Genetics • Original Paper
Structural analysis
Subpopulations
Wilt
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Summary:Safflower ( Carthamus tinctorius L.), an oilseed crop, is severely affected by Fusarium oxysporum f. sp. carthami (Foc), a fungus causing Fusarium wilt (FW) resulting in up to 80% yield loss. In the present study, we used a panel of 84 diverse accessions from the composite core collection to perform association mapping for FW-resistance. Hydroponics-based screening resulted in categorization of 84 accessions as 31 immune, 19 highly resistant, 9 moderately resistant, 4 moderately susceptible, and 21 highly susceptible. Genotyping with a combination of 155 AFLP and 144 SSR markers revealed substantial genetic differentiation and structure analysis identified three main subpopulations ( K  = 3) with nearly 35% of admixtures in the panel. Kinship analysis at individual and population level revealed absence of or weak relatedness between the accessions. Association mapping with General Linear Model and Mixed Linear Model identified 4 marker-trait associations (MTAs) significantly linked with the FW-resistance trait. Of these, 3 robust MTAs identified in both the models exhibited phenotypic variance ranging from 4.09 to 6.45%. Locus-128 showing a low P- value and high phenotypic variance was identified as a promising marker-trait association that will facilitate marker-assisted breeding for FW-resistance in safflower.
ISSN:1234-1983
2190-3883
DOI:10.1007/s13353-022-00694-z