Evaluation of elastic change during the mitotic phase of murine breast cancer cells using scanning acoustic microscopy

Abstract Scanning acoustic microscopy (SAM) is a useful observational tool in cellular study as non-invasive living observation is feasible, unlike in conventional optical microscopy. In a previous study, cell morphological changes were successfully visualized using acoustic impedance measurements....

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2022-07, Vol.61 (SG), p.SG1070
Main Authors: Soon, Thomas Tiong Kwong, Sasaki, Ruka, Prastika, Edo Bagus, Kawaguchi, Yuki, Kobayashi, Kazuto, Hozumi, Naohiro, Yoshida, Sachiko
Format: Article
Language:English
Subjects:
Acoustic impedance
Acoustic microscopes
c-mode
cancer cell
cell culture
Evaluation
living cell observation
Mitosis
Optical microscopy
Scanning acoustic microscopy
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Summary:Abstract Scanning acoustic microscopy (SAM) is a useful observational tool in cellular study as non-invasive living observation is feasible, unlike in conventional optical microscopy. In a previous study, cell morphological changes were successfully visualized using acoustic impedance measurements. These acoustic impedance changes correspond to cell elasticity, mainly reflecting changes in the cytoskeleton. In this study, we evaluate the elastic changes in murine breast cancer cell C127I during mitosis. C127I cells were cultured to ∼75% confluency before measurement, using a transducer with a central frequency of 320 MHz. Dynamic changes during mitosis were successfully mapped using SAM and confirmed by laser confocal microscopy. Cells in prometaphase, anaphase, and telophase, which could previously only be confirmed through immunostaining, were successfully visualized using SAM. This suggests that SAM is capable of distinguishing cells in different mitotic phases based on the changes in acoustic impedance.
ISSN:0021-4922
1347-4065
DOI:10.35848/1347-4065/ac54f7