Investigation of the Oxidative Stress Response of a Green Synthesis Nanoparticle (RP-Ag/ACNPs) in Zebrafish

Silver nanoparticles (AgNPs) are prominent nanomaterials that are efficiently used in different industries including medical products, water treatment, and cosmetics. However, AgNPs are known to cause adverse effects on the ecosystem and human health. In this study, aqueous extract of Rumex patienti...

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Bibliographic Details
Published in:Biological trace element research 2022-06, Vol.200 (6), p.2897-2907
Main Authors: Kokturk, Mine, Yıldırım, Serkan, Nas, Mehmet Salih, Ozhan, Gunes, Atamanalp, Muhammed, Bolat, Ismail, Calimli, Mehmet Harbi, Alak, Gonca
Format: Article
Language:English
Subjects:
Activated carbon
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cosmetics
Danio rerio
Defects
Degeneration
Dosage
Edema
Electron microscopy
Embryos
Freshwater fishes
Hatching
Industry
Larvae
Leukocytes (neutrophilic)
Life Sciences
Medical equipment
Morphology
Mortality causes
Nanomaterials
Nanoparticles
Nanotechnology
Necrosis
Neurodegeneration
Nutrition
Oncology
Oxidative stress
Silver
Spinal curvature
Stabilizers (agents)
Stabilizing
Survival
Toxicity tests
Transmission electron microscopy
Water treatment
X-ray diffraction
Yolk sac
Zebrafish
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Summary:Silver nanoparticles (AgNPs) are prominent nanomaterials that are efficiently used in different industries including medical products, water treatment, and cosmetics. However, AgNPs are known to cause adverse effects on the ecosystem and human health. In this study, aqueous extract of Rumex patientia (RP) was used as a reducing and stabilizing agent in AgNP biosynthesis. The obtained activated carbon (AC) from Chenopodium album (CA) plant was combined with RP-AgNPs to synthesize RP-Ag/AC NPs. Next, the effects of these green synthesis RP-Ag/AC NPs on zebrafish ( Danio rerio ) embryos and larvae were investigated. First, we characterized the RP-Ag/AC NPs by using X-ray diffraction (XRD) and transmission electron microscopy (TEM) and determined LC 50 value as 217.23 mg/L at 96 h. Next, the alterations in survival rate, hatching rate, and morphology of the larvae at 96 h were monitored. The survival rates decreased in a dose-dependent manner. Morphological defects such as yolk sac edema, pericardial edema, spinal curvature, and tail malformation in the NP-treated larvae were observed. RP-Ag/AC NPs stimulated the production of neuronal NOS (nNOS) and 8-OHdG in zebrafish brain tissues in a dose-dependent manner and enhanced neutrophil degeneration and necrosis at concentrations of 50 and 100 mg/L. Thus, the obtained data suggest that the green synthesis process is not sufficient to reduce the effect of oxidative stress caused by AgNPs on oxidative signaling.
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-021-02855-3