Virus-induced silencing of a tobacco deoxyhypusine synthase gene

A cDNA fragment corresponding to deoxyhypusine synthase gene NbDHS was isolated and cloned into potato virus X （PVX） vector for functional analysis in Nico. tiana benthamiana by using virus-induced gene silencing （VIGS）. Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increa...

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Bibliographic Details
Published in:Chinese science bulletin 2005-12, Vol.50 (23), p.2707-2713
Main Authors: Hongzhi, Wang, Rongcai, Ma, Ruifen, Li, Guoying, Wang, Jianhua, Wei
Format: Article
Language:English
Subjects:
Chlorophyll
Flowering
Flowers & plants
Functional analysis
Gene silencing
Genetic modification
Leaves
Plant growth
Polymerase chain reaction
Senescence
Tobacco
Viruses
基因克隆
生长促进作用
脱氧羟腐胺缩赖氨酸合酶基因
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Summary:A cDNA fragment corresponding to deoxyhypusine synthase gene NbDHS was isolated and cloned into potato virus X （PVX） vector for functional analysis in Nico. tiana benthamiana by using virus-induced gene silencing （VIGS）. Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level of DHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS in N. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance of DHS and eIF-5A, we concluded that NbDHS plays important roles in plant growth, development and senescence. The possible application of DHS gene in genetic modification of crops and horticultural plants was discussed.
ISSN:1001-6538
2095-9273
1861-9541
2095-9281
DOI:10.1007/BF02899640