Poly(ethyleneoxide-block-methylmethacrylate) derived recessed nanodisc electrode array as an ultrasensitive electrochemical sensing platform for immunosensor

In this work, we present the synthesis of amphiphilic diblock copolymer poly(ethylene oxide-block-methylmethacrylate) (PEO-b-PMMA) using atom transfer radical polymerization and demonstrate its application for the fabrication of recessed nanodisk-array electrode (RNE) as a platform for protein detec...

Full description

Saved in:
Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2022-03, Vol.354, p.131240, Article 131240
Main Authors: Nathani, Akash, Qi, Yinghua, Varshney, Sunil K., Sharma, Chandra Shekhar
Format: Article
Language:English
Subjects:
Arrays
Atomic force microscopy
Biomarkers
Biosensor
Block copolymers
Chemical synthesis
Coated electrodes
Direct immersion annealing
Electrodes
Ethylene oxide
Glassy carbon
Immunosensors
Mesoporous thin film
Nanoelectrode array
PEO-b-PMMA
Phase separation
Polymethyl methacrylate
Proteins
Selectivity
Serum albumin
Silicon wafers
Spin coating
Thin films
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, we present the synthesis of amphiphilic diblock copolymer poly(ethylene oxide-block-methylmethacrylate) (PEO-b-PMMA) using atom transfer radical polymerization and demonstrate its application for the fabrication of recessed nanodisk-array electrode (RNE) as a platform for protein detection. For the fabrication of thin-film, PEO-b-PMMA was spin-coated on the silicon wafer and the phase separation was achieved by a unique direct immersion annealing technique followed by washing with the water/methanol solution to remove the PEO phase. The microphase separation and comprehensive porosity characterizations were done using AFM and electron microscopy. Later, the thin film was loaded on the surface of the glassy carbon electrode by wedge transfer technique and the RNE property of the mesoporous thin film was confirmed using cyclic voltammetry (CV). For the protein sensing application, the thin film-coated electrode was biotinylated using EDC-NHS chemistry, followed by the blocking of the non-specific sites using bovine serum albumin (BSA). The reduction in the CV current and the increase in the channel resistance in EIS at each stage of fabrication confirmed the successful immobilization. For the streptavidin detection, the RNE displayed an ultra-low detection limit of 0.15 fg/ml with enhanced selectivity which confirms the potential of PEO-b-PMMA based RNE as an ultrasensitive immunosensor platform for any biomarker detection. •Direct immersion annealing is useful in assisting the microphase separation.•PEO-b-PMMA avails facial generation of porosity and biofunctionalization.•Wedge transfer is effective technique allowing the use of any desired base electrode.•PEO-b-PMMA based RNE provides an ultra-low limit of detection.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2021.131240