In vitro propagation of Vaccinium floribundum Kunth from seeds: promissory technology for mortiño accelerated production

The Andean mortiño (Vaccinium floribundum Kunth) grows wild in the northern paramos of South América. The berries present potential opportunities for agribusiness, but an efficient technology for the production of plants and berries is required. On the other hand, the development of plant production...

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Bibliographic Details
Published in:Canadian journal of plant science 2022-02, Vol.102 (1), p.216-224
Main Authors: Meneses, L.S, Morillo, L.E, Vásquez-Castillo, W
Format: Article
Language:English
Subjects:
Acclimatization
Acetic acid
Adenine
Andean paramos
Auxin
Auxins
Culture media
Cytokinins
Fruits
Germination
Greenhouses
Indole-3-butyric acid
Indoleacetic acid
Micropropagation
mortiño
Naphthaleneacetic acid
Organic acids
Paramos
paramos Andin
Peat
Photoperiods
plant production
Plant sciences
Production processes
production végétale
Propagation
Rooting
Saturated fatty acids
Seed germination
Seed industry
Seedlings
Seeds
semences
Technology application
Vaccinium floribundum
Zeatin riboside
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Summary:The Andean mortiño (Vaccinium floribundum Kunth) grows wild in the northern paramos of South América. The berries present potential opportunities for agribusiness, but an efficient technology for the production of plants and berries is required. On the other hand, the development of plant production methods from mortiño seeds will allow the plants’ accessibility and its potential use in breeding programs. The objective of this study was to develop an efficient in vitro protocol for accelerated production using seeds. We carried out the research in four phases: seed germination, plant multiplication, rooting, and acclimatization in the greenhouse. For in vitro seed germination, we studied the effect of two culture media [woody plant medium (WPM) and Murashige and Skoog medium (MS], two photoperiods (16 and 24 hours of light), and two temperatures (18 and 28 °C). The best treatment was found to be WPM + 24 h light + 18 °C. In the micropropagation of seedlings, the effect of two concentrations of three cytokinines [(trans-zeatin riboside (TZR), zeatin (ZEA), and 2-isopentenyl adenine (2iP)], and two photoperiods (16 and 0 h light) was evaluated, whereby the concentration of 0.5 mg L−1 of TZR was the best treatment. For rooting, two doses of three auxins [indole-3-butyric acid (IBA), 1-naphthaleneacetic acid (NAA), and indole-3-acetic acid (IAA)] were evaluated, resulting in the 2 mg·L−1 concentration of IBA giving the best root induction. Finally, the in vitro rooted plants were acclimatized in a greenhouse. We found that peat was the best substrate. These results show that the technology developed here is useful for in vitro production of V. floribundum using seeds.
ISSN:0008-4220
1918-1833
DOI:10.1139/CJPS-2020-0290