Synthesis and characterization of a stable and inert MnII-based ZnII responsive MRI probe for molecular imaging of glucose stimulated zinc secretion (GSZS)

A new potential ZnII responsive MnII-based MRI CA candidate derived from pyclen-3,9-diacetate (3,9-PC2A) has been synthesized and it possesses a di-(2-picolyl)amine (DPA) moiety as an active arm. The PC2A-DPA ligand was found to form a thermodynamically stable complex with MnII (log KMnL = 15.87(6)...

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Bibliographic Details
Published in:Inorganic chemistry frontiers 2022-01, Vol.9 (3), p.577-583
Main Authors: Botár, Richárd, Molnár, Enikő, Garda, Zoltán, Madarasi, Enikő, Trencsényi, György, Kiss, János, Kálmán, Ferenc K, Tircsó, Gyula
Format: Article
Language:English
Subjects:
Glucose
Inorganic chemistry
Magnetic resonance imaging
Serum albumin
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Summary:A new potential ZnII responsive MnII-based MRI CA candidate derived from pyclen-3,9-diacetate (3,9-PC2A) has been synthesized and it possesses a di-(2-picolyl)amine (DPA) moiety as an active arm. The PC2A-DPA ligand was found to form a thermodynamically stable complex with MnII (log KMnL = 15.87(6) which translates into a pMn of 8.79) which also possesses outstanding inertness towards ZnII ion triggered transmetallation (t1/2 at pH = 6.0 is 64.5 h). The [Mn(PC2A-DPA)] complex has a relatively high relaxation rate (3.11 s−1 at 1.41 T and 37 °C for a 0.96 mM complex) which is not affected by the ZnII concentration directly, yet in the co-presence of 0.7 mM human serum albumin (HSA) the relaxation rate values (both R1 and R2) increased significantly (9.08 s−1 at 1.41 T and 37 °C, for a 0.748 mM complex) owing to the efficient binding of the complex to the protein (log Kaff = 3.5 ± 0.1 M−1). The increase is large enough to visualize it by MR imaging using phantoms in vitro while preliminary in vivo MRI experiments confirmed that the glucose stimulated ZnII secretion (GSZS) can be visualized in the prostate of a healthy mouse.
ISSN:2052-1545
2052-1553
DOI:10.1039/d1qi00501d