Ethylenediamine grafted carbon nanotube aerogels modified screen-printed electrode for simultaneous electrochemical immunoassay of multiple tumor markers

•Hybrid carbon aerogels were synthesized and introduced for immunosensor application.•The sandwich configuration immunosensor was based on the screen-printed technology.•Redox mediators (dyes) have been used as labels.•The improved immunosensor was employed for individual and simultaneous electroche...

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Bibliographic Details
Published in:Journal of electroanalytical chemistry (Lausanne, Switzerland) Switzerland), 2021-11, Vol.900, p.115700, Article 115700
Main Authors: Filik, Hayati, Avan, Asiye Aslıhan, Altaş Puntar, Nilay, Özyürek, Mustafa, Güngör, Zeynep Banu, Kucur, Mine, Kamış, Handan, Dicle, Diclehan Ali
Format: Article
Language:English
Subjects:
Aerogels
Alpha-fetoprotein
Antibodies
Antigens
Carcinoembryonic antigen
Electrochemistry
Ethylenediamine
Fourier transforms
Immunoassay
Immunosensor
Immunosensors
Infrared analysis
Multi wall carbon nanotubes
Safranine
Sandwich structures
Serum analysis
Square waves
Tumors
Voltammetry
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Summary:•Hybrid carbon aerogels were synthesized and introduced for immunosensor application.•The sandwich configuration immunosensor was based on the screen-printed technology.•Redox mediators (dyes) have been used as labels.•The improved immunosensor was employed for individual and simultaneous electrochemical detection of AFP and CEA.•The developed EC sensor provided a sufficient linear range of 0.005–1.0 ng/mL for AFP and CEA. A sensitive sandwich-model immunoassay designed for simultaneous electrochemical measurement of alpha-fetoprotein and carcinoembryonic antigen handling multiple-label strategy. The sandwich-model immunosensor was constructed by assembling ethylenediamine-MWCNT aerogels (EDA-CAGs), AuNPs and capture antibodies (Cp-Ab1) on the screen-printed carbon electrode (SPCE). The prepared EDA-CAG textures were analyzed using Fourier transform infrared (FT-IR), Scanning Electron Microscopy (SEM) and Energy Dispersive X-Ray Spectroscopy (EDX). In this protocol, AuNPs decorated EDA-CAG-carried Thionine (Thi) and AuNPs decorated EDA-CAG-carried Safranine O (SfO) (denoted as AuNP-Thi-EDA-CAG and AuNP-SfO-EDA-CAG, respectively) were properly exploited as distinguishable signal labels, which were used to attach detection antibodies (Ab2) (anti-AFP2 and anti-CEA2), respectively. When two tumor antigens were present, a square wave voltammetry (SWV) scan displayed two well-separated signals, each signal indicated one target antigen. Observed results introduced that the square wave voltammetric peak current exhibited a good linear relationship to logarithm concentration in the ranges from 0.005 to 1.0 ng/mL for both analytes. The LoDs were 0.0015 and 0.0010 ng/mL (at signal/noise S/N = 3) for AFP and CEA, respectively. The designed sandwich-type immunosensor was applied to real serum sample analysis.
ISSN:1572-6657
1873-2569
DOI:10.1016/j.jelechem.2021.115700