Genetic diversity and pathogenicity of potato spindle tuber viroid and chrysanthemum stunt viroid isolates in Russia

To investigate the current status of viroid infection in potato fields in Russia, potato spindle tuber viroid (PSTVd) and chrysanthemum stunt viroid (CSVd) were detected in potato ( Solanum tuberosum ) and S. nigrum plants growing in the Volga (V), North-Western (NW), and Far Eastern (FE) federal di...

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Bibliographic Details
Published in:European journal of plant pathology 2021-11, Vol.161 (3), p.529-542
Main Authors: Matsushita, Yosuke, Yanagisawa, Hironobu, Khiutti, Aleksandr, Mironenko, Nina, Ohto, Yasuo, Afanasenko, Olga
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Ecology
Gene sequencing
Genetic diversity
Genomes
Infectivity
Life Sciences
Nucleotides
Pathogenicity
Pathogens
Phylogeny
Plant Pathology
Plant Sciences
Potatoes
Solanum nigrum
Solanum tuberosum
Spindles
Stunt
Tomatoes
Vegetables
Viroids
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Summary:To investigate the current status of viroid infection in potato fields in Russia, potato spindle tuber viroid (PSTVd) and chrysanthemum stunt viroid (CSVd) were detected in potato ( Solanum tuberosum ) and S. nigrum plants growing in the Volga (V), North-Western (NW), and Far Eastern (FE) federal districts in Russia. While both PSTVd and CSVd were detected in potato samples from the V and FE regions, only CSVd was detected in potato samples from NW. CSVd was detected in S. nigrum from FE, and this was the first detection of CSVd in S. nigrum . Analysis of the complete genomes of 30 PSTVd and 10 CSVd variants revealed that 5 and 7 PSTVd variants from V and FE, respectively, and 2, 4, and 3 CSVd variants from V, NW, and FE, respectively, were new. Analysis of the infectivity of and symptoms caused by 15 Russian PSTVd isolates in tomato revealed that 14 PSTVd isolates caused intermediate or severe symptoms, and only FP14 caused mild symptoms. Our survey revealed that most of the Russian PSTVd isolates from V and FE caused intermediate symptoms in tomato, and the genome sequences were identical to that of PSTVd isolated from S. demissum. Phylogenetic analysis of nucleotide sequences of the Russian PSTVd variants showed their close relationship to previously studied PSTVd isolates, suggesting that most strains in Russia have not substantially changed genetically, but have widely spread in potato fields.
ISSN:0929-1873
1573-8469
DOI:10.1007/s10658-021-02339-z