Extraction of Antioxidant Compounds from Blueberry Fruit Waste and Evaluation of Their In Vitro Biological Activity in Human Keratinocytes (HaCaT)

The purpose of this study was to investigate the biological properties of an extract obtained from the waste of blueberry fruit. The study covered the optimization of extraction of antioxidants from blueberry pomace and the determination of antioxidant properties of the extract using HaCaT as the mo...

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Bibliographic Details
Published in:Food analytical methods 2021-11, Vol.14 (11), p.2317-2327
Main Authors: Piechowiak, Tomasz, Skóra, Bartosz, Grzelak-Błaszczyk, Katarzyna, Sójka, Michał
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anthocyanins
Antioxidants
Biological activity
Biological properties
Blueberries
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Dietary supplements
Ethanol
Food Science
Fruits
Functional foods & nutraceuticals
Hydrogen peroxide
Keratinocytes
Mathematical models
Microbiology
Optimization
Oxidative stress
Polyphenols
Sustainable development
Waste materials
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Summary:The purpose of this study was to investigate the biological properties of an extract obtained from the waste of blueberry fruit. The study covered the optimization of extraction of antioxidants from blueberry pomace and the determination of antioxidant properties of the extract using HaCaT as the model organism. Research showed that the yield of antioxidants extraction from blueberry waste was dependent on the applied extraction conditions. Based on the mathematical models, the optimal conditions of extraction process in which the maximum quantity of antioxidant compounds is achieved from the waste mass unit, i.e., the relation of the waste mass to the volume of ethanol equal to 1:17.36, and process time equal to 1000 s. The obtained extract was characterized by high antioxidant activity, which was shaped by high content of polyphenols, mainly anthocyanins. Moreover, the extract showed a high ability to protect HaCaT cells from the occurrence of oxidative stress induced by H 2 O 2 . Cells treated with the extract and H 2 O 2 generated a lower amount of ROS than cells treated with H 2 O 2 only. The obtained results will be base of further studies on applying the extract in production of diet supplements and functional foods with increased antioxidant activity. Moreover, the main research material is blueberry pomace which is a troublesome waste material for juice producers. Consequently, according to a sustainable development idea, the study results will provide an opportunity to increase interest in the problem of rational use of the waste material to a certain extent.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-021-02056-7