Fabrication and evaluation of aptamer-conjugated paclitaxel-loaded magnetic nanoparticles for targeted therapy on breast cancer cells

Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier...

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Bibliographic Details
Published in:Molecular biology reports 2021-03, Vol.48 (3), p.2105-2116
Main Authors: Khodadadi, Emad, Mahjoub, Soleiman, Arabi, Mehdi Sheikh, Najafzadehvarzi, Hossein, Nasirian, Vahid
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Antitumor agents
Aptamers
Aptamers, Nucleotide - chemistry
Biomedical and Life Sciences
Breast cancer
Breast Neoplasms - drug therapy
Breast Neoplasms - pathology
Cancer
Cell Line, Tumor
Cell Survival
Drug delivery
Drug Liberation
Endocytosis
Female
Fluorescence
Histology
Humans
Infrared spectroscopy
Inhibitory Concentration 50
Internalization
Life Sciences
Light scattering
Magnetite Nanoparticles - chemistry
Magnetite Nanoparticles - ultrastructure
Mice
Molecular Targeted Therapy
Morphology
Nanoparticles
Oligonucleotides
Original Article
Paclitaxel
Paclitaxel - therapeutic use
Particle Size
Reproducibility of Results
Spectroscopy, Fourier Transform Infrared
Static Electricity
Thermogravimetry
Transmission electron microscopy
X-Ray Diffraction
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Summary:Targeted drug delivery vehicles make it possible to deliver anti-cancer drugs to the cells or tissues of interest. Aptamers are peptide or oligonucleotide molecules that can serve as targeting elements of drug carriers. In the current study, we evaluated the capacity of an aptamer-based drug carrier to deliver Paclitaxel (PTX) to cancer cells. After being synthesized, SPIONs@PTX-SYL3C aptamer was characterized using different methods, including differential light scattering (DLS), infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), X-ray diffraction (XRD), Thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM). Encapsulation efficiency (EE) and loading efficiency (LE) were also evaluated. The carrier was applied on 4T1, MCF 7, and MCF-10A breast cell lines to evaluate its drug delivery potency and specificity. EE and LE were calculated to be 77.6% and 7.76%, respectively. MTT results revealed that aptameric SPIONs@PTX was more toxic than non-aptameric SPIONs@PTX. Flowcytometry analysis and DAPI staining confirmed that SPIONs@PTX-Aptamer had higher cell internalization rate when compared to non-targeted SPIONs@PTX. Our results indicate that aptamer-conjugated SPIONs@PTX has a good capacity in recognizing its target cells and inhibiting their growth and division.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06199-y