Preparation of a Novel pH-responsive Fluorescent Probe Based on an Imidazo[1,2-a]indole Fluorophore and its Application in Detecting Extremely Low pH in Saccharomyces cerevisiae

A novel pH-responsive probe based on an imidazo[1,2-a]indole fluorophore architecture is reported. The probe was highly selective to strongly acidic pH (p K a = 3.56) with high sensitivity and a fast response time (within 30 s). The probe did not demonstrate any fluorescence changes in the presence...

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Bibliographic Details
Published in:Journal of fluorescence 2021-09, Vol.31 (5), p.1219-1225
Main Authors: Xu, Yanhao, Duan, Ruikang, Liu, Hao, Xia, Chengcai, Duan, Guiyun, Ge, Yanqing
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biomedicine
Biophysics
Biotechnology
Charge transfer
Fluorescent Dyes
Fluorescent indicators
Fungi
HeLa Cells
Humans
Hydrogen-Ion Concentration
Indoles
Rapid Communication
Response time
Saccharomyces cerevisiae
Yeast
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Summary:A novel pH-responsive probe based on an imidazo[1,2-a]indole fluorophore architecture is reported. The probe was highly selective to strongly acidic pH (p K a = 3.56) with high sensitivity and a fast response time (within 30 s). The probe did not demonstrate any fluorescence changes in the presence of interfering metal ions, and it featured excellent reversibility under strongly acidic conditions. The mechanism of detection of the probe was determined to be based on intramolecular charge transfer (ICT) at different pH. The probe was also able to be used for imaging for detecting acidic pH in Saccharomyces cerevisiae .
ISSN:1053-0509
1573-4994
DOI:10.1007/s10895-021-02739-8