Ploidy level, epigenetic and in vitro environment influence the indirect somatic embryogenesis of the new synthetic autoallohexaploid Coffea

Autoallohexaploid plantlets of “Híbrido de Timor” (HT, Coffea ) were recently regenerated by chromosome set doubling from allotriploid HT. Besides the in vitro propagation and chromosome doubling set, indirect somatic embryogenesis allows us to investigate the short-term polyploidy consequences. We...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2021-09, Vol.146 (3), p.553-563
Main Authors: de Morais Oliveira, João Paulo, Sanglard, Natália Arruda, Ferreira, Adésio, Clarindo, Wellington Ronildo
Format: Article
Language:English
Subjects:
Activated carbon
Activated charcoal
Biomedical and Life Sciences
Callus
Charcoal
Chromosomes
Embryonic growth stage
Embryos
Environments
Epigenetics
Explants
Friability
Germplasm
Hybrids
Karyotypes
Life Sciences
Original Article
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plantlets
Ploidy
Polyploidy
Propagation
Regeneration
Somatic embryogenesis
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Summary:Autoallohexaploid plantlets of “Híbrido de Timor” (HT, Coffea ) were recently regenerated by chromosome set doubling from allotriploid HT. Besides the in vitro propagation and chromosome doubling set, indirect somatic embryogenesis allows us to investigate the short-term polyploidy consequences. We aimed to establish and compare the indirect somatic embryogenesis in natural allotriploid and synthetic autoallohexaploid HT, evaluating the role of the in vitro environment, ploidy level and global level of 5-methylcytosine on somatic embryo regeneration and karyotype stability. Our results showed that the autoallohexaploid needed more time for induction and proliferation of friable calli, showing lower mean numbers of responsive explants and a higher mean level of 5-methylcytosine. Autoallohexaploid also showed a higher 5-methylcytosine level during the somatic embryo regeneration, but both hybrids exhibited the same mean value of mature cotyledonary somatic embryos for friable callus. Regarding the in vitro environment, the activated charcoal at 8 and 16 g L −1 increased global level of 5-methylcytosine and the mean number of abnormal somatic embryo in autoallohexaploid. Based on these results, the autoallohexaploid exhibited a lower in vitro response compared to its allotriploid ancestor, despite having at least twice as many copies of in vitro response genes. Besides, our data evidenced that the in vitro environment, ploidy level and global 5-methylcytosine level influence the Coffea indirect somatic embryogenesis, showing the outcome of the short-term induced polyploidy. Therefore, we showed the euploidy influence during indirect somatic embryogenesis, demonstrating the relevance of the “omics” evaluations of the new Coffea germplasm in relation to their ancestor. Key message In vitro environment, ploidy level and global 5-methylcytosine level influence the Coffea indirect somatic embryogenesis, evidencing the outcome of the short-term induced polyploidy.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-021-02093-4