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Dietary RNAi toxicity assay suggests α and γ subunits of HvCOPI as novel molecular targets for Henosepilachna vigintioctopunctata, an emerging coccinellid pest
Henosepilachna vigintioctopunctata is a highly polyphagous pest of solanaceous plants in Asia and has been predominantly controlled using synthetic insecticides. The intensive use of insecticides has led to the development of resistance, creating an urgent need for new control strategies, for this i...
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Published in: | Journal of pest science 2021-09, Vol.94 (4), p.1473-1486 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Henosepilachna vigintioctopunctata
is a highly polyphagous pest of solanaceous plants in Asia and has been predominantly controlled using synthetic insecticides. The intensive use of insecticides has led to the development of resistance, creating an urgent need for new control strategies, for this insect pest. RNA interference (RNAi) has been explored as a pest management strategy in multiple insect pests. In this study, we explored RNAi as a potential alternative for controlling
H. vigintioctopunctata.
Based on preliminary research, we hypothesized that
HvCOPI
, a coat protein complex that facilitates retrograde transport, is a promising novel molecular target for
H. vigintioctopunctata
control. To test this overarching hypothesis, we examined the toxicity of double-stranded RNAs (dsRNAs) towards
H. vigintioctopunctata
using dietary RNAi toxicity assays targeting eight
HvCOPI
subunits, including
α-, β-, β
′
-, γ-, δ-, ε-, ζ-
, and
Arf1-COPI
using in vitro synthesized and bacterially expressed dsRNAs. The results demonstrated that ingestion of
dsHvCOPIs
induced acute feeding cessation in both larvae and adults, which led to significant mortality. In this study,
H. vigintioctopunctata
showed similar sensitivity to both types of
dsHvCOPIs
production. Ingestion of bacterially expressed
dsHvαCOPI
and
dsHvγCOPI
led to the highest mortality in both larvae and adults. Interestingly, there was a significant positive relationship between the knockdown efficiency of different
HvCOPI
subunits and the corresponding mortality on day 2 and 4, respectively. These combined results suggest that
α
and
γ
subunits of
HvCOPI
are promising targets for the development of RNAi-based biopesticides for the management of
H. vigintioctopunctata
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ISSN: | 1612-4758 1612-4766 |
DOI: | 10.1007/s10340-021-01350-x |