Arecoline induces heart injure via Fas/Fas ligand apoptotic pathway in heart of Sprague–Dawley rat

Habitual chewing of areca nut increases the risk of cardiovascular disease mortality, but less report demonstrate the toxic mechanism of areca nut on heart. To investigate toxicity of areca nut on cardiomyocytes, we induced the heart injury with arecoline to evaluate the acute damage of areca nut on...

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Published in:Environmental toxicology 2021-08, Vol.36 (8), p.1567-1575
Main Authors: Lin, Wen‐Yuan, Tsai, Bruce Chi‐Kang, Day, Cecilia Hsuan, Chiu, Ping‐Ling, Chen, Ray‐Jade, Chen, Michael Yu‐Chih, Padma, V. Vijaya, Luk, Hsiang‐Ning, Lee, Hsiang‐Chen, Huang, Chih‐Yang
Format: Article
Language:English
Subjects:
Apoptosis
Areca catechu
areca nut
arecoline
betel nut
cardiac toxicity
Cardiomyocytes
Cardiovascular diseases
Chewing
Dosage
Fas/Fas ligand
FasL protein
Health risks
Heart
heart damage
Injuries
Injury analysis
Mitochondria
Nuts
Peritoneum
Proteins
Receptors
Toxicity
Western blotting
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Summary:Habitual chewing of areca nut increases the risk of cardiovascular disease mortality, but less report demonstrate the toxic mechanism of areca nut on heart. To investigate toxicity of areca nut on cardiomyocytes, we induced the heart injury with arecoline to evaluate the acute damage of areca nut on heart. Different concentrations of are coline (lowdosage: 5 mg/kg/day and high dosage 50 mg/kg/day) were injected into Sprague‐Dawley rat via intra‐peritoneal method for 21 days to create negative effects of arecoline on cardiomyocyte. Themyocardial architecture of the rat heart was observed. The arecoline‐induced apoptotic proteins were analysed via western blotting. The myocardialarchitecture of heart was injured with arecoline and TUNEL stain was also shown are coline‐induced cardiac apoptosis. Arecoline promoted the protein expression of both Fas dependent snd mitochondrial dependent apoptosis. In summary, arecoline induces cardiac toxicity and apoptosis by inducing both death receptor and mitochondria‐dependent apoptotic pathways on heart.
ISSN:1520-4081
1522-7278
DOI:10.1002/tox.23153