A biocompatible dual-AIEgen system without spectral overlap for quantitation of microbial viability and monitoring of biofilm formation

The lack of rapid and reliable microbial detection and sensing platforms and insufficient understanding of microbial behavior may delay precautions that could be made, which is a great threat to human life and increases the heavy financial burden on society. In this contribution, a dual-aggregation-...

Full description

Saved in:
Bibliographic Details
Published in:Materials horizons 2021-06, Vol.8 (6), p.1816-1824
Main Authors: He, Wei, Zheng, Zheng, Bai, Haotian, Xiong, Ling-Hong, Wang, Lei, Li, Yinghui, Kwok, Ryan T. K, Lam, Jacky W. Y, Hu, Qinghua, Cheng, Jinquan, Tang, Ben Zhong
Format: Article
Language:English
Subjects:
Biocompatibility
Biofilms
Boronic Acids
Fluorescence
Fluorescent Dyes
Humans
Metabolism
Microbial Viability
Microorganisms
Morphology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The lack of rapid and reliable microbial detection and sensing platforms and insufficient understanding of microbial behavior may delay precautions that could be made, which is a great threat to human life and increases the heavy financial burden on society. In this contribution, a dual-aggregation-induced emission luminogen (AIEgen) system is successfully developed for microbial imaging and metabolic status sensing. This system consists of two AIEgens (DCQA and TPE-2BA) that bear positively charged groups or boronic acid groups, providing universal microbial staining ability and specific affinity for dead microbes, respectively. Based on the distinctive fluorescence response produced by the diverse interaction of AIEgens with live or dead microbes, this dual-AIEgen system can detect all the microbes and identify their viabilities. Furthermore, the morphology and metabolic status of a sessile biofilm can also be imaged and monitored. The system exhibits rapid labelling properties that suitable for various microbes, and good biocompatibilities. A dual-AIEgen system for microbial imaging and metabolic status sensing has been realized through chemistry strategies. This dual-AIEgen system can detect general microbes and identify their viabilities as well as their microbial biofilms.
ISSN:2051-6347
2051-6355
DOI:10.1039/d1mh00149c