Quantitative and chemical fingerprint analysis for quality control of Zingiber zerumbet based on HPTLC combined with chemometric methods

A simple, reliable high-performance thin-layer chromatography (HPTLC) method was developed for chemical fingerprinting of Zingiber zerumbet and quantitative estimation of zerumbone. Thirty-six batches of Z. zerumbet were collected from five eco-regions of eastern India. Zerumbone content varied from...

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Bibliographic Details
Published in:Plant biosystems 2021-08, Vol.155 (4), p.711-720
Main Authors: Dash, Biswabhusan, Ray, Asit, Sahoo, Ambika, Jena, Sudipta, Singh, Subhashree, Kar, Basudeba, Patnaik, Jeetendranath, Panda, Pratap Chandra, Mohanty, Sujata, Nayak, Sanghamitra
Format: Article
Language:English
Subjects:
Aquatic plants
Chemical fingerprinting
Chemometrics
Chromatographic fingerprinting
Cluster analysis
Coniferous forests
Deciduous forests
Fingerprinting
Forests
hierarchical clustering analysis
principal component analysis
Principal components analysis
Quality assessment
Quality control
Rhizomes
Stability tests
Thin layer chromatography
Zerumbone
Zingiber zerumbet
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Summary:A simple, reliable high-performance thin-layer chromatography (HPTLC) method was developed for chemical fingerprinting of Zingiber zerumbet and quantitative estimation of zerumbone. Thirty-six batches of Z. zerumbet were collected from five eco-regions of eastern India. Zerumbone content varied from 52.4 to 214.6 mg/g (dry weight) in methanolic extract of Z. zerumbet rhizomes. Zerumbone content was in the following order: moist deciduous forests of the lower Gangetic plains > Brahmaputra valley evergreen forest > Odisha semi-evergreen forests > Sundarbans freshwater swamp forests > moist deciduous forest of the eastern highlands. Relative Standard Deviation (RSD) of the relative peak areas (RPA) and relative retention times (RRT) of eight characteristic peaks in repeatability and stability test were
ISSN:1126-3504
1724-5575
DOI:10.1080/11263504.2020.1779840