Cryopreservation of sperm from the brain coral Diploria labyrinthiformis as a strategy to face the loss of corals in the Caribbean

In this study, we evaluated the efficacy of sperm cryopreservation for use in larval-based propagation of Diploria labyrinthiformis and produced offspring that were maintained under controlled conditions. Gametes were collected from colonies in situ in July and August 2017 and 2018 . The four larges...

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Bibliographic Details
Published in:Coral reefs 2021-06, Vol.40 (3), p.937-950
Main Authors: Grosso-Becerra, M. V., Mendoza-Quiroz, S., Maldonado, E., Banaszak, A. T.
Format: Article
Language:English
Subjects:
Biological fertilization
Biomedical and Life Sciences
Colonies
Controlled conditions
Corals
Cryopreservation
Diploria labyrinthiformis
Eggs
Fertilization
Freshwater & Marine Ecology
Gametes
Genetic crosses
Larvae
Life Sciences
Motility
Nursery grounds
Oceanography
Offspring
Polyps
Polyps (organisms)
Recruitment (fisheries)
Spawning
Sperm
Spermatozoa
Survival
Symbionts
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Summary:In this study, we evaluated the efficacy of sperm cryopreservation for use in larval-based propagation of Diploria labyrinthiformis and produced offspring that were maintained under controlled conditions. Gametes were collected from colonies in situ in July and August 2017 and 2018 . The four largest colonies out of a total of nine appear to be senescent or produce low-quality sperm or eggs. Sperm was cryopreserved for comparison of the effects of storage time on sperm viability. We determined that cryopreserved sperm from D. labyrinthiformis is viable for at least 13 months for use in in vitro crosses, though their motility is reduced on average by 24% in comparison with fresh sperm. Using frozen sperm to fertilize freshly collected eggs led to successful fertilization, larval yields, settlement and post-settlement survival. In general, these were lower by 23%, 23%, 14% and 8%, respectively, when compared to controls fertilized with fresh sperm. Our results suggest that motility of fresh sperm is not a good indicator of the future fate of larvae because in some cases low motility led to successful settlement. We also found that not all crosses were successful, and that the direction of the cross significantly affects larval yields and settlement. Once symbionts were noticeable within the primary polyps the cryo-recruits were maintained in an ex situ nursery for observation and showed similar survival with respect to recruits produced with fresh sperm. Prior to the 2018 spawning event, Stony Coral Tissue Loss Disease (SCTLD) was detected in the studied colonies and by February 2020 seven of the nine colonies (78%) had succumbed to the disease. The sperm from these colonies was banked in a repository and since then has been used in genetic rescue projects for this species. Thus, we show that cryopreservation is a useful tool in actions designed to recover D. labyrinthiformis and can potentially be applied to other species of corals severely affected by SCTLD or in need of genetic rescue.
ISSN:0722-4028
1432-0975
DOI:10.1007/s00338-021-02098-7